Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 288625..289256 Replicon chromosome
Accession NZ_CP082355
Organism Escherichia coli strain VCSW5

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag K7X33_RS01245 Protein ID WP_001260301.1
Coordinates 288625..288900 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag K7X33_RS01250 Protein ID WP_000593555.1
Coordinates 288897..289256 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X33_RS01230 (283629) 283629..284696 + 1068 WP_000361509.1 HlyD family secretion protein -
K7X33_RS01235 (284693) 284693..287428 + 2736 WP_000149112.1 ribosome-associated ATPase/putative transporter RbbA -
K7X33_RS01240 (287428) 287428..288552 + 1125 WP_001309020.1 ABC transporter permease -
K7X33_RS01245 (288625) 288625..288900 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
K7X33_RS01250 (288897) 288897..289256 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K7X33_RS01255 (289376) 289376..289777 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
K7X33_RS01260 (289783) 289783..290589 - 807 WP_000173652.1 nickel import ATP-binding protein NikE -
K7X33_RS01265 (290586) 290586..291350 - 765 WP_001136200.1 nickel import ATP-binding protein NikD -
K7X33_RS01270 (291350) 291350..292183 - 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
K7X33_RS01275 (292180) 292180..293124 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T214668 WP_001260301.1 NZ_CP082355:288625-288900 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T214668 NZ_CP110170:323185-323553 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT214668 WP_000593555.1 NZ_CP082355:288897-289256 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT214668 NZ_CP110170:322967-323188 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References