Detailed information of TA system TA214666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 11197..11719
• Replicon: plasmid pVCSW10.2
• Accession: NZ_CP082353
• Organism: Providencia rettgeri strain VCSW10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K7H99_RS21555
• Protein ID: WP_222961159.1
• Coordinates: 11438..11719 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K7H99_RS21550
• Protein ID: WP_222961158.1
• Coordinates: 11197..11448 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H99_RS21520 (K7H99_21520)	7050..7322	-	273	WP_222961148.1	cytoplasmic protein	-
K7H99_RS21525 (K7H99_21525)	7352..7984	-	633	WP_222961150.1	recombinase family protein	-
K7H99_RS21530 (K7H99_21530)	8179..8421	+	243	WP_222961152.1	type II toxin-antitoxin system ParD family antitoxin	-
K7H99_RS21535 (K7H99_21535)	8414..8719	+	306	WP_222961153.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K7H99_RS21540 (K7H99_21540)	9005..10387	-	1383	WP_222961154.1	PepSY-associated TM helix domain-containing protein	-
K7H99_RS21545 (K7H99_21545)	10396..10833	-	438	WP_222961155.1	DUF2946 domain-containing protein	-
K7H99_RS21550 (K7H99_21550)	11197..11448	+	252	WP_222961158.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K7H99_RS21555 (K7H99_21555)	11438..11719	+	282	WP_222961159.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H99_RS21560 (K7H99_21560)	12339..13115	+	777	WP_222961161.1	MBL fold metallo-hydrolase	-
K7H99_RS21565 (K7H99_21565)	13131..14117	+	987	Protein_12	helix-turn-helix domain-containing protein	-
K7H99_RS21575 (K7H99_21575)	14334..15067	-	734	Protein_13	IS6 family transposase	-
K7H99_RS21585 (K7H99_21585)	15137..15733	-	597	WP_282565799.1	flavin reductase family protein	-
K7H99_RS21590 (K7H99_21590)	15792..16649	-	858	WP_222961166.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56282	56282
	-	flank	IS/Tn	-	-	7352..7921	569
	-	flank	IS/Tn	-	-	14435..15067	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10978.93 Da
• Isoelectric Point: 10.6516

>T214666 WP_222961159.1 NZ_CP082353:11438-11719 [Providencia rettgeri]
MIYKLEFDKRALKEWQKLAPPIRNQFKKKLAERLENPFVPSAQLSGKHNRYKIKLRSLGYRLVYEVVDEKIVLLVIAVGK
REGNAIYFSSNER

Nucleotide

• Length: 282 bp

>T214666 NZ_CP110168:c175911-175600 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9101.30 Da
• Isoelectric Point: 3.7939

>AT214666 WP_222961158.1 NZ_CP082353:11197-11448 [Providencia rettgeri]
MTYQILSDTAASITDLKKNPMGTIAGGEGNPVAILNRNEPAFYCVPPELFAWFMELAEDVELNKVADERLQSLDAVSVNI
DDL

Nucleotide

• Length: 252 bp

>AT214666 NZ_CP110168:c175603-175184 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCATATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA