Detailed information of TA system TA214665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 138958..139465
• Replicon: plasmid pVCSW10.1
• Accession: NZ_CP082352
• Organism: Providencia rettgeri strain VCSW10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K7H99_RS21015
• Protein ID: WP_222960756.1
• Coordinates: 139190..139465 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K7H99_RS21010
• Protein ID: WP_282565750.1
• Coordinates: 138958..139203 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H99_RS22310	134539..134664	+	126	Protein_137	IS3 family transposase	-
K7H99_RS22475	135705..135881	-	177	WP_282565749.1	hypothetical protein	-
K7H99_RS20990 (K7H99_20990)	136057..136503	+	447	WP_222960754.1	hypothetical protein	-
K7H99_RS21000 (K7H99_21000)	136635..136975	-	341	Protein_140	transposase	-
K7H99_RS21005 (K7H99_21005)	137872..138045	+	174	Protein_141	transposase	-
K7H99_RS21010 (K7H99_21010)	138958..139203	+	246	WP_282565750.1	DUF6290 family protein	Antitoxin
K7H99_RS21015 (K7H99_21015)	139190..139465	+	276	WP_222960756.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H99_RS21020 (K7H99_21020)	140526..140831	-	306	WP_222960759.1	nitroreductase family protein	-
K7H99_RS21025 (K7H99_21025)	140835..141179	-	345	WP_222960761.1	nitroreductase family protein	-
K7H99_RS21030 (K7H99_21030)	142148..143382	-	1235	Protein_146	IS30 family transposase	-
K7H99_RS21035 (K7H99_21035)	143849..144079	-	231	WP_222960777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..243713	243713
	-	flank	IS/Tn	-	-	136706..136975	269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (7-70)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10957.89 Da
• Isoelectric Point: 10.3778

>T214665 WP_222960756.1 NZ_CP082352:139190-139465 [Providencia rettgeri]
MPHYRIEYTKRFQKELKKLGRCHQKIIQSYIEKNIDNSINPRLIGKILKGDKAEFIRYRIGDYRLIATIEDERLVILALS
IGHRKDVYKHF

Nucleotide

• Length: 276 bp

>T214665 NZ_CP110168:168133-168549 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9188.30 Da
• Isoelectric Point: 4.2182

>AT214665 WP_282565750.1 NZ_CP082352:138958-139203 [Providencia rettgeri]
MRKNLTKAIRLNNEELSLFESYANAKGLTFSELCKSAIIEKIEDEIDLKLAEEAYEEYLNDNETLSHKDIFDPLGNYASL

Nucleotide

• Length: 246 bp

>AT214665 NZ_CP110168:167906-168136 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA