Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 62639..63186 Replicon plasmid pAL.H8
Accession NZ_CP082350
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag K7H97_RS23205 Protein ID WP_001384452.1
Coordinates 62908..63186 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag K7H97_RS23200 Protein ID WP_021546940.1
Coordinates 62639..62908 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS23175 (K7H97_23175) 58073..58174 + 102 WP_001326870.1 NAD(P)-binding domain-containing protein -
K7H97_RS23180 (K7H97_23180) 58200..59168 - 969 WP_004099053.1 IS5-like element IS903B family transposase -
K7H97_RS23485 59350..59478 + 129 WP_001334698.1 hypothetical protein -
K7H97_RS23185 (K7H97_23185) 59767..60024 + 258 WP_001333498.1 helix-turn-helix transcriptional regulator -
K7H97_RS23190 (K7H97_23190) 60099..60686 + 588 WP_001333497.1 GNAT family N-acetyltransferase -
K7H97_RS23195 (K7H97_23195) 60952..61656 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
K7H97_RS23200 (K7H97_23200) 62639..62908 + 270 WP_021546940.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
K7H97_RS23205 (K7H97_23205) 62908..63186 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
K7H97_RS23210 (K7H97_23210) 63232..63444 + 213 Protein_56 3'-5' exonuclease -
K7H97_RS23215 (K7H97_23215) 63539..63775 + 237 WP_223417154.1 transposase -
K7H97_RS23220 (K7H97_23220) 64024..64575 + 552 Protein_58 3'-5' exonuclease -
K7H97_RS23225 (K7H97_23225) 64744..64919 + 176 Protein_59 integrase core domain-containing protein -
K7H97_RS23230 (K7H97_23230) 64992..65678 + 687 WP_023281065.1 DNA-binding transcriptional regulator rspR -
K7H97_RS23235 (K7H97_23235) 65775..66224 + 450 Protein_61 transposase -
K7H97_RS23240 (K7H97_23240) 66474..67454 - 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ugd / galF / gnd 1..79616 79616
- inside IScluster/Tn - - 58200..78825 20625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T214660 WP_001384452.1 NZ_CP082350:62908-63186 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T214660 NZ_CP110167:c4643469-4642936 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT214660 WP_021546940.1 NZ_CP082350:62639-62908 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT214660 NZ_CP110167:c4643746-4643480 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References