214657

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4088108..4089401 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name sanaT Uniprot ID A0A3Z8A2R8
Locus tag K7H97_RS19955 Protein ID WP_000342496.1
Coordinates 4088108..4089013 (-) Length 302 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag K7H97_RS19960 Protein ID WP_000152741.1
Coordinates 4089006..4089401 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS19925 (4083919) 4083919..4084635 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
K7H97_RS19930 (4084655) 4084655..4085761 + 1107 WP_001044128.1 N-acetylneuraminate epimerase -
K7H97_RS19935 (4085826) 4085826..4086806 + 981 WP_001366032.1 sialate O-acetylesterase -
K7H97_RS19940 (4086814) 4086814..4087464 - 651 WP_001037970.1 HNH endonuclease -
K7H97_RS19945 (4087601) 4087601..4087744 + 144 Protein_3907 HNH endonuclease -
K7H97_RS19950 (4087843) 4087843..4088028 + 186 WP_000066585.1 hypothetical protein -
K7H97_RS19955 (4088108) 4088108..4089013 - 906 WP_000342496.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
K7H97_RS19960 (4089006) 4089006..4089401 - 396 WP_000152741.1 DUF6088 family protein Antitoxin
K7H97_RS19965 (4089471) 4089471..4090304 - 834 WP_001192742.1 DUF4942 domain-containing protein -
K7H97_RS19970 (4090610) 4090610..4092166 + 1557 WP_024232412.1 type I restriction-modification system subunit M -
K7H97_RS19975 (4092163) 4092163..4093368 + 1206 WP_001292892.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimE / fimB 4080787..4093368 12581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-300)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 302 a.a.        Molecular weight: 34012.91 Da        Isoelectric Point: 4.7207

>T214657 WP_000342496.1 NZ_CP082349:c4089013-4088108 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYEMRYQQFVSPMVYGERKITWDEA

Download         Length: 906 bp

>T214657 NZ_CP110167:1872562-1872664 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT214657 WP_000152741.1 NZ_CP082349:c4089401-4089006 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT214657 NZ_CP110167:c1872671-1872526 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Z8A2R8

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References