Detailed information of TA system TA214655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3527407..3528244
• Replicon: chromosome
• Accession: NZ_CP082349
• Organism: Escherichia coli strain AL.H8

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: K7H97_RS17335
• Protein ID: WP_000227784.1
• Coordinates: 3527702..3528244 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: K7H97_RS17330
• Protein ID: WP_001297137.1
• Coordinates: 3527407..3527718 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H97_RS17305 (3522427)	3522427..3523374	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
K7H97_RS17310 (3523396)	3523396..3525387	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K7H97_RS17315 (3525377)	3525377..3525991	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K7H97_RS17320 (3525991)	3525991..3526320	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K7H97_RS17325 (3526332)	3526332..3527222	+	891	WP_000971336.1	heme o synthase	-
K7H97_RS17330 (3527407)	3527407..3527718	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
K7H97_RS17335 (3527702)	3527702..3528244	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
K7H97_RS17340 (3528300)	3528300..3529235	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
K7H97_RS17345 (3529643)	3529643..3531007	+	1365	WP_001000960.1	MFS transporter	-
K7H97_RS17350 (3531135)	3531135..3531626	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K7H97_RS17355 (3531794)	3531794..3532705	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T214655 WP_000227784.1 NZ_CP082349:3527702-3528244 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T214655 NZ_CP110167:337238-337606 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT214655 WP_001297137.1 NZ_CP082349:3527407-3527718 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT214655 NZ_CP110167:337020-337241 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3