214654

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3493330..3493948 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K7H97_RS17165 Protein ID WP_001291435.1
Coordinates 3493730..3493948 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K7H97_RS17160 Protein ID WP_000344800.1
Coordinates 3493330..3493704 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS17150 (3488419) 3488419..3489612 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K7H97_RS17155 (3489635) 3489635..3492784 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K7H97_RS17160 (3493330) 3493330..3493704 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K7H97_RS17165 (3493730) 3493730..3493948 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K7H97_RS17170 (3494119) 3494119..3494670 + 552 WP_000102564.1 maltose O-acetyltransferase -
K7H97_RS17175 (3494786) 3494786..3495256 + 471 WP_000136192.1 YlaC family protein -
K7H97_RS17180 (3495420) 3495420..3496970 + 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K7H97_RS17185 (3497012) 3497012..3497365 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K7H97_RS17195 (3497744) 3497744..3498055 + 312 WP_000409911.1 MGMT family protein -
K7H97_RS17200 (3498086) 3498086..3498658 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214654 WP_001291435.1 NZ_CP082349:3493730-3493948 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214654 NZ_CP110163:24189-24470 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214654 WP_000344800.1 NZ_CP082349:3493330-3493704 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214654 NZ_CP110163:23948-24199 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References