Detailed information of TA system TA214652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2502688..2503326
• Replicon: chromosome
• Accession: NZ_CP082349
• Organism: Escherichia coli strain AL.H8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K7H97_RS12300
• Protein ID: WP_000813794.1
• Coordinates: 2503150..2503326 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K7H97_RS12295
• Protein ID: WP_001270285.1
• Coordinates: 2502688..2503104 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H97_RS12275 (2497840)	2497840..2498781	-	942	WP_001251315.1	ABC transporter permease	-
K7H97_RS12280 (2498782)	2498782..2499795	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
K7H97_RS12285 (2499813)	2499813..2500958	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
K7H97_RS12290 (2501203)	2501203..2502609	-	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
K7H97_RS12295 (2502688)	2502688..2503104	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K7H97_RS12300 (2503150)	2503150..2503326	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K7H97_RS12305 (2503548)	2503548..2503778	+	231	WP_000494244.1	YncJ family protein	-
K7H97_RS12310 (2503870)	2503870..2505831	-	1962	WP_223042296.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K7H97_RS12315 (2505904)	2505904..2506440	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K7H97_RS12320 (2506532)	2506532..2507707	+	1176	WP_001236268.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2507747..2508895	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T214652 WP_000813794.1 NZ_CP082349:c2503326-2503150 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T214652 NZ_CP110161:c133283-132801 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT214652 WP_001270285.1 NZ_CP082349:c2503104-2502688 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT214652 NZ_CP110161:c133552-133274 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCCCAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• PDB: 6HPB