Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1373014..1373639 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag K7H97_RS06790 Protein ID WP_000911329.1
Coordinates 1373241..1373639 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag K7H97_RS06785 Protein ID WP_000450524.1
Coordinates 1373014..1373241 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS06760 (1368816) 1368816..1369286 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
K7H97_RS06765 (1369286) 1369286..1369858 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
K7H97_RS06770 (1370004) 1370004..1370882 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
K7H97_RS06775 (1370899) 1370899..1371933 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
K7H97_RS06780 (1372146) 1372146..1372859 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
K7H97_RS06785 (1373014) 1373014..1373241 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
K7H97_RS06790 (1373241) 1373241..1373639 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
K7H97_RS06795 (1373786) 1373786..1374649 + 864 WP_001267498.1 neutral zinc metallopeptidase -
K7H97_RS06800 (1374664) 1374664..1376679 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
K7H97_RS06805 (1376753) 1376753..1377451 + 699 WP_000679823.1 esterase -
K7H97_RS06810 (1377561) 1377561..1377761 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T214640 WP_000911329.1 NZ_CP082349:1373241-1373639 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T214640 NZ_CP110160:851699-852109 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT214640 WP_000450524.1 NZ_CP082349:1373014-1373241 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT214640 NZ_CP110160:851452-851718 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References