Detailed information of TA system TA214634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 65883..66913
• Replicon: plasmid pLsch
• Accession: NZ_CP082348
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: K7H94_RS22390
• Protein ID: WP_222952519.1
• Coordinates: 65883..66458 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: K7H94_RS22395
• Protein ID: WP_222952520.1
• Coordinates: 66455..66913 (-)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS22375 (K7H94_22375)	62499..62639	-	141	Protein_76	type II toxin-antitoxin system YhaV family toxin	-
K7H94_RS22385 (K7H94_22385)	64840..65874	+	1035	WP_222952518.1	tyrosine-type recombinase/integrase	-
K7H94_RS22390 (K7H94_22390)	65883..66458	-	576	WP_222952519.1	PIN domain-containing protein	Toxin
K7H94_RS22395 (K7H94_22395)	66455..66913	-	459	WP_222952520.1	helix-turn-helix domain-containing protein	Antitoxin
K7H94_RS22400 (K7H94_22400)	67374..67661	-	288	WP_222952521.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K7H94_RS22405 (K7H94_22405)	67658..67909	-	252	WP_222952522.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K7H94_RS22410 (K7H94_22410)	68705..69634	+	930	WP_222952523.1	type VI secretion system ImpA family N-terminal domain-containing protein	-
K7H94_RS22415 (K7H94_22415)	69657..70196	+	540	WP_222952524.1	type VI secretion system contractile sheath small subunit	-
K7H94_RS22420 (K7H94_22420)	70199..71719	+	1521	WP_222952525.1	type VI secretion system contractile sheath large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181041	181041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• SinI-like, DNA-binding domain (81-123)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21377.43 Da
• Isoelectric Point: 5.0346

>T214634 WP_222952519.1 NZ_CP082348:c66458-65883 [Pantoea dispersa]
MSSGYTVIFDACVLSPAPLRSFLMHLAAGGQFRARCTEDIHEEWIRNVLLNRTELNRAQLQRVRALMDRHVPDALVTGYQ
SLTESIRGLPDEDDRHVVAAAIVAQAEGIVTFNLKDFPDDVLATWNLRAIHSDSFITDLTELDIAVVLDAARRQRASLKS
PPFTPDEFLDCLLSQQLPETVSRLRPLTALI

Nucleotide

• Length: 576 bp

>T214634 NZ_CP110154:c87854-87438 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGTTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGCCATCCGGATAGCGCAGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 16984.47 Da
• Isoelectric Point: 6.7243

>AT214634 WP_222952520.1 NZ_CP082348:c66913-66455 [Pantoea dispersa]
MTQLQNKPPSLQEAKIARLCSRELSAVLETRAESQQVSLTDKSGRIHNVSIPVSALRMLVDVLTELGNGNAVRLVPVGAE
MTTQEVADLLNISRPTLIRLLDNDELPYHRAGNRRKIRYVDALAYREKLMNQRHDALVELGALDQELGLGYE

Nucleotide

• Length: 459 bp

>AT214634 NZ_CP110154:c88081-87851 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA