Detailed information of TA system TA214632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 621258..621853
• Replicon: plasmid unnamed
• Accession: NZ_CP082347
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: K7H94_RS21620
• Protein ID: WP_222952209.1
• Coordinates: 621258..621635 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: K7H94_RS21625
• Protein ID: WP_088517422.1
• Coordinates: 621632..621853 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS21600 (K7H94_21600)	616840..617187	-	348	WP_021508880.1	helix-turn-helix transcriptional regulator	-
K7H94_RS21605 (K7H94_21605)	617347..618573	+	1227	WP_222952207.1	MFS transporter	-
K7H94_RS21610 (K7H94_21610)	618627..619991	-	1365	WP_058759247.1	H(+)/Cl(-) exchange transporter ClcA	-
K7H94_RS21615 (K7H94_21615)	620328..621251	+	924	WP_222952208.1	glutaminase B	-
K7H94_RS21620 (K7H94_21620)	621258..621635	-	378	WP_222952209.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K7H94_RS21625 (K7H94_21625)	621632..621853	-	222	WP_088517422.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K7H94_RS21630 (K7H94_21630)	622145..622561	+	417	WP_222952210.1	DUF2946 domain-containing protein	-
K7H94_RS21635 (K7H94_21635)	622627..624366	+	1740	WP_222952211.1	DUF2534 family protein	-
K7H94_RS21640 (K7H94_21640)	624447..625463	-	1017	WP_021508873.1	NAD(P)-dependent alcohol dehydrogenase	-
K7H94_RS21645 (K7H94_21645)	625587..625856	-	270	WP_010616678.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..689940	689940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13977.92 Da
• Isoelectric Point: 6.9891

>T214632 WP_222952209.1 NZ_CP082347:c621635-621258 [Pantoea dispersa]
MNHVSADEVIALHDYVLRRYAGVTGMQDPGRAEAIVTRVINRQFYEGVQDVYELAATYWVAISRGHIFADANKRTSLNVT
MLFLKRNGVKVHDRPELVELTVMAATGEAGIDDLARALRSFFSAH

Nucleotide

• Length: 378 bp

>T214632 NZ_CP110154:6702-7172 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTACCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7999.82 Da
• Isoelectric Point: 4.5026

>AT214632 WP_088517422.1 NZ_CP082347:c621853-621632 [Pantoea dispersa]
MNKFTYTSARENFSQVLDQAIAGEAVEITRRSGDSAVVISKAHFDAYRKAALDAEFAELIGEFDASNQALADR

Nucleotide

• Length: 222 bp

>AT214632 NZ_CP110154:6262-6705 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA