Detailed information of TA system TA214627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3823506..3824088
• Replicon: chromosome
• Accession: NZ_CP082346
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: K7H94_RS17825
• Protein ID: WP_058771045.1
• Coordinates: 3823789..3824088 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A8E1S1N8
• Locus tag: K7H94_RS17820
• Protein ID: WP_058775497.1
• Coordinates: 3823506..3823808 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS17815 (K7H94_17815)	3820120..3823500	+	3381	WP_222951418.1	cellulose synthase complex outer membrane protein BcsC	-
K7H94_RS17820 (K7H94_17820)	3823506..3823808	-	303	WP_058775497.1	BrnA antitoxin family protein	Antitoxin
K7H94_RS17825 (K7H94_17825)	3823789..3824088	-	300	WP_058771045.1	BrnT family toxin	Toxin
K7H94_RS17830 (K7H94_17830)	3824311..3825315	-	1005	WP_222951419.1	glycosyl hydrolase family 8	-
K7H94_RS17835 (K7H94_17835)	3825325..3825771	-	447	WP_058758083.1	cellulose biosynthesis protein BcsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (9-90)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (10-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11919.36 Da
• Isoelectric Point: 6.9910

>T214627 WP_058771045.1 NZ_CP082346:c3824088-3823789 [Pantoea dispersa]
MQTEPEFEWDTTKAETNFRKHGIRFEVAARVFEDPFHLSVQDRYENGEYRWQTIGNVMGHVVILVAHTVRFETDVERIRI
ISARHATRIERRRYEQSQV

Nucleotide

• Length: 300 bp

>T214627 NZ_CP110153:4149147-4149365 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11490.15 Da
• Isoelectric Point: 9.6528

>AT214627 WP_058775497.1 NZ_CP082346:c3823808-3823506 [Pantoea dispersa]
MSKVRFKRSELPPLTDKQQAELDALSRKQDSDIQCDDIAPLDDSFWQAAERGRFYRPVKTQASIRIDADILEWLKAPGKG
YQTRLNAILRAAMLRELQGK

Nucleotide

• Length: 303 bp

>AT214627 NZ_CP110153:4148746-4149120 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG