Detailed information of TA system TA214622

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 634294..635069
• Replicon: chromosome
• Accession: NZ_CP082346
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: K7H94_RS02980
• Protein ID: WP_045141661.1
• Coordinates: 634698..635069 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K7H94_RS02975
• Protein ID: WP_045141662.1
• Coordinates: 634294..634659 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS02940 (K7H94_02940)	630115..631002	+	888	WP_222951617.1	50S ribosome-binding GTPase	-
K7H94_RS02945 (K7H94_02945)	631185..631583	+	399	WP_045141668.1	hypothetical protein	-
K7H94_RS02950 (K7H94_02950)	631640..632104	+	465	WP_045141667.1	hypothetical protein	-
K7H94_RS02955 (K7H94_02955)	632202..633023	+	822	WP_045141666.1	DUF932 domain-containing protein	-
K7H94_RS02960 (K7H94_02960)	633055..633495	+	441	WP_045141665.1	antirestriction protein	-
K7H94_RS02965 (K7H94_02965)	633508..634050	+	543	WP_045141664.1	DNA repair protein RadC	-
K7H94_RS02970 (K7H94_02970)	634047..634268	+	222	WP_045141663.1	DUF987 family protein	-
K7H94_RS02975 (K7H94_02975)	634294..634659	+	366	WP_045141662.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K7H94_RS02980 (K7H94_02980)	634698..635069	+	372	WP_045141661.1	TA system toxin CbtA family protein	Toxin
K7H94_RS02985 (K7H94_02985)	635584..636462	+	879	WP_222951618.1	AraC family transcriptional regulator	-
K7H94_RS02990 (K7H94_02990)	636606..636935	+	330	WP_058757234.1	DUF1493 family protein	-
K7H94_RS02995 (K7H94_02995)	636973..637752	-	780	WP_215481540.1	NAD(P)H-dependent oxidoreductase	-
K7H94_RS03000 (K7H94_03000)	637843..638733	+	891	WP_222951619.1	LysR family transcriptional regulator	-
K7H94_RS03005 (K7H94_03005)	638748..639239	-	492	WP_058780921.1	DUF4411 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13848.89 Da
• Isoelectric Point: 7.0930

>T214622 WP_045141661.1 NZ_CP082346:634698-635069 [Pantoea dispersa]
MQTLSSHLTRAAKSCLSPVEIWQRLLTHLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRSDHKTVTRVTAGHHQEL

Nucleotide

• Length: 372 bp

>T214622 NZ_CP110153:705271-705612 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13417.06 Da
• Isoelectric Point: 4.5884

>AT214622 WP_045141662.1 NZ_CP082346:634294-634659 [Pantoea dispersa]
MNNHSESGTKPENPACQQWGLQSTITPCFGARLVQEGNCLHFLADRAGFNGAFSDDEALHLDQAFLLILKQLELMLTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYIAIYPDQIEFQ

Nucleotide

• Length: 366 bp

>AT214622 NZ_CP110153:704933-705250 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA