Detailed information of TA system TA214620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 586465..587060
• Replicon: plasmid unnamed
• Accession: NZ_CP082342
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: K7X52_RS22570
• Protein ID: WP_088517421.1
• Coordinates: 586465..586842 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: K7X52_RS22575
• Protein ID: WP_088517422.1
• Coordinates: 586839..587060 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS22545 (K7X52_22545)	581539..581886	-	348	WP_021508880.1	helix-turn-helix transcriptional regulator	-
K7X52_RS22550 (K7X52_22550)	582046..583272	+	1227	WP_222986886.1	MFS transporter	-
K7X52_RS22555 (K7X52_22555)	583482..583760	+	279	WP_222986888.1	helix-turn-helix transcriptional regulator	-
K7X52_RS22560 (K7X52_22560)	583834..585198	-	1365	WP_247653161.1	H(+)/Cl(-) exchange transporter ClcA	-
K7X52_RS22565 (K7X52_22565)	585535..586458	+	924	WP_166719384.1	glutaminase B	-
K7X52_RS22570 (K7X52_22570)	586465..586842	-	378	WP_088517421.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K7X52_RS22575 (K7X52_22575)	586839..587060	-	222	WP_088517422.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K7X52_RS22580 (K7X52_22580)	587352..587768	+	417	WP_222986889.1	DUF2946 domain-containing protein	-
K7X52_RS22585 (K7X52_22585)	587834..589573	+	1740	WP_222986891.1	DUF2534 family protein	-
K7X52_RS22590 (K7X52_22590)	589654..590670	-	1017	WP_021508873.1	NAD(P)-dependent alcohol dehydrogenase	-
K7X52_RS22595 (K7X52_22595)	590794..591063	-	270	WP_010616678.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..653898	653898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13976.94 Da
• Isoelectric Point: 7.4656

>T214620 WP_088517421.1 NZ_CP082342:c586842-586465 [Pantoea dispersa]
MNHVSADEVIALHDYVLRRYAGVTGMQDPGRAQAIVTRVINRQFYEGVQDVYELAATYWVAISRGHIFADANKRTSLNVT
MLFLKRNGVKVHDRPELVELTVMAATGEAGIDDLARALRSFFSAH

Nucleotide

• Length: 378 bp

>T214620 NZ_CP110151:c81277-80852 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7999.82 Da
• Isoelectric Point: 4.5026

>AT214620 WP_088517422.1 NZ_CP082342:c587060-586839 [Pantoea dispersa]
MNKFTYTSARENFSQVLDQAIAGEAVEITRRSGDSAVVISKAHFDAYRKAALDAEFAELIGEFDASNQALADR

Nucleotide

• Length: 222 bp

>AT214620 NZ_CP110151:c81504-81274 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA