Detailed information of TA system TA214619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 567270..567847
• Replicon: plasmid unnamed
• Accession: NZ_CP082342
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K7X52_RS22470
• Protein ID: WP_222986874.1
• Coordinates: 567515..567847 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8E1V6C0
• Locus tag: K7X52_RS22465
• Protein ID: WP_058770344.1
• Coordinates: 567270..567515 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS22445 (K7X52_22445)	562632..563585	+	954	WP_222986871.1	carbohydrate kinase family protein	-
K7X52_RS22450 (K7X52_22450)	563589..564671	+	1083	WP_058776100.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
K7X52_RS22455 (K7X52_22455)	564791..566200	-	1410	WP_058758997.1	MFS transporter	-
K7X52_RS22460 (K7X52_22460)	566316..567170	+	855	WP_222986872.1	helix-turn-helix transcriptional regulator	-
K7X52_RS22465 (K7X52_22465)	567270..567515	+	246	WP_058770344.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K7X52_RS22470 (K7X52_22470)	567515..567847	+	333	WP_222986874.1	endoribonuclease MazF	Toxin
K7X52_RS22475 (K7X52_22475)	567953..568126	+	174	WP_021508895.1	hypothetical protein	-
K7X52_RS22480 (K7X52_22480)	568156..568503	+	348	WP_021508894.1	hypothetical protein	-
K7X52_RS22485 (K7X52_22485)	568543..569736	-	1194	WP_222986875.1	zinc-dependent alcohol dehydrogenase	-
K7X52_RS22490 (K7X52_22490)	570000..570707	+	708	WP_021508892.1	phage antirepressor KilAC domain-containing protein	-
K7X52_RS22495 (K7X52_22495)	570742..570957	-	216	WP_021508891.1	hypothetical protein	-
K7X52_RS22500 (K7X52_22500)	571099..572280	-	1182	WP_222986877.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..653898	653898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11972.78 Da
• Isoelectric Point: 7.8577

>T214619 WP_222986874.1 NZ_CP082342:567515-567847 [Pantoea dispersa]
MVSRFVPDAGDLIWLDFDPTLGHEQGGHRPAVVLSPFAYNNKVGMLLCVPCTTQVKGYPFEVSLTGSRDSVALADQVTSI
DWRARKVVKKSNVTADELSEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T214619 NZ_CP110151:40128-40439 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8955.25 Da
• Isoelectric Point: 4.5580

>AT214619 WP_058770344.1 NZ_CP082342:567270-567515 [Pantoea dispersa]
MTTVTLKKWGNSPSIRIPSSVMQSASLSVDDKLELSVEDGKLILVPLREKVYSLDALLDEITDDNVHDKIDFGPSVGKER
I

Nucleotide

• Length: 246 bp

>AT214619 NZ_CP110151:40436-40855 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA