Detailed information of TA system TA214615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 64424..64958
• Replicon: plasmid unnamed
• Accession: NZ_CP082342
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: K7X52_RS20205
• Protein ID: WP_222986984.1
• Coordinates: 64424..64714 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A246NFI2
• Locus tag: K7X52_RS20210
• Protein ID: WP_058758675.1
• Coordinates: 64704..64958 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS20175 (K7X52_20175)	60033..60266	-	234	WP_222986980.1	hypothetical protein	-
K7X52_RS20180 (K7X52_20180)	60324..61004	-	681	WP_031279936.1	O-methyltransferase	-
K7X52_RS20185 (K7X52_20185)	61098..61736	+	639	WP_104187797.1	TetR family transcriptional regulator	-
K7X52_RS20190 (K7X52_20190)	61872..62693	+	822	WP_021508741.1	Cof-type HAD-IIB family hydrolase	-
K7X52_RS20195 (K7X52_20195)	62780..63208	-	429	WP_222986981.1	helix-turn-helix domain-containing protein	-
K7X52_RS20200 (K7X52_20200)	63317..64366	+	1050	WP_222986983.1	NAD(P)-dependent alcohol dehydrogenase	-
K7X52_RS20205 (K7X52_20205)	64424..64714	-	291	WP_222986984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7X52_RS20210 (K7X52_20210)	64704..64958	-	255	WP_058758675.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K7X52_RS20215 (K7X52_20215)	65183..67018	+	1836	WP_222986986.1	methyl-accepting chemotaxis protein	-
K7X52_RS20220 (K7X52_20220)	67192..68268	+	1077	WP_021508737.1	diguanylate cyclase	-
K7X52_RS20225 (K7X52_20225)	68269..68730	-	462	WP_104093520.1	OsmC family protein	-
K7X52_RS20230 (K7X52_20230)	68859..69629	-	771	WP_021508734.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..653898	653898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11203.09 Da
• Isoelectric Point: 10.4241

>T214615 WP_222986984.1 NZ_CP082342:c64714-64424 [Pantoea dispersa]
MTYKLKFVPSAEKEWKKLGHPVREQFKKKLAQRLENPRVPSAQLHGRKDQYKIKLRASGYRLVYLVQDETITVMVLGVGK
REGSQVYVDTECRVPE

Nucleotide

• Length: 291 bp

>T214615 NZ_CP110150:c4633948-4633664 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9288.50 Da
• Isoelectric Point: 3.9370

>AT214615 WP_058758675.1 NZ_CP082342:c64958-64704 [Pantoea dispersa]
MPFQILSNTAASITDLKRDPMGTVRSAEGETIAILNRNEPAFYCVPPAVYQYLMELAEDAELGRIADERISELADAKEIS
LDDL

Nucleotide

• Length: 255 bp

>AT214615 NZ_CP110150:c4634180-4633938 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A246NFI2