Detailed information of TA system TA214614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4036514..4037096
• Replicon: chromosome
• Accession: NZ_CP082341
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: K7X52_RS18975
• Protein ID: WP_222985936.1
• Coordinates: 4036797..4037096 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A8E1S1N8
• Locus tag: K7X52_RS18970
• Protein ID: WP_058775497.1
• Coordinates: 4036514..4036816 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS18965 (K7X52_18965)	4033128..4036508	+	3381	WP_222985935.1	cellulose synthase complex outer membrane protein BcsC	-
K7X52_RS18970 (K7X52_18970)	4036514..4036816	-	303	WP_058775497.1	BrnA antitoxin family protein	Antitoxin
K7X52_RS18975 (K7X52_18975)	4036797..4037096	-	300	WP_222985936.1	BrnT family toxin	Toxin
K7X52_RS18980 (K7X52_18980)	4037319..4038323	-	1005	WP_031279620.1	glycosyl hydrolase family 8	-
K7X52_RS18985 (K7X52_18985)	4038333..4038779	-	447	WP_058758083.1	cellulose biosynthesis protein BcsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (9-90)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (10-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11991.42 Da
• Isoelectric Point: 6.6474

>T214614 WP_222985936.1 NZ_CP082341:c4037096-4036797 [Pantoea dispersa]
MQTEPEFEWDTTKAETNFRKHGIRFEVAARVFEDPFHLSVQDRYENGEYRWQTIGNVMEHVVILVAHTVRFETDVERIRI
ISARHATRIERRRYEQSQV

Nucleotide

• Length: 300 bp

>T214614 NZ_CP110150:c4548969-4548628 [Klebsiella pneumoniae]
ATGAATACTTTACCTGTAATAAACCAGCGGGCTATTCAGTTATGCCCATCGCCTGTAACTATCTGGCAAACGTTGCTAAC
TCGTTTACTGGAACAACACTACGGGCTGGCGCTGAACGACACACCGTTCGGTGATGAAAGCGTGATTCAGGAACATATCG
AGGCCGGTATTACACTCGTTGATGCCGTTAACTTCTTGGTGGAAAAGTACGAGCTGGTTCGTATCGACCGCTGGGCGTTT
GGCTGGTTGGAACCATCTCCATACTTGCGAGCTGAAGACATTCTGCGAATGCGTCGGGACATGGGGCTGTTGCGAGGCTG
CAACCACACTGCAGCAATGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11490.15 Da
• Isoelectric Point: 9.6528

>AT214614 WP_058775497.1 NZ_CP082341:c4036816-4036514 [Pantoea dispersa]
MSKVRFKRSELPPLTDKQQAELDALSRKQDSDIQCDDIAPLDDSFWQAAERGRFYRPVKTQASIRIDADILEWLKAPGKG
YQTRLNAILRAAMLRELQGK

Nucleotide

• Length: 303 bp

>AT214614 NZ_CP110150:c4549354-4549004 [Klebsiella pneumoniae]
ATGTCGAATAAGACACAGACAGTGAATGGTGATACTGCCGAGCCCAGGTGGGGCTTGAGTTGTAACGTCATCCCTTGTTT
TGGTGCACGTCTGGTCCAGGAAGGTAACCGCCTACATTATCTGGCTGACCGCGCCAGCATAACAGGTCAGTTCAATGAAG
CGGATTTAATCCATCCTGACCAGGCATTCCCGGTCCTGTTGAAGCAGGCAGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACGTTCTGTGAAAAGGGGTTGACCTGCGAAGCTGATACGCTGGGTTCCTGTGGCCACGT
CTATATTGTCATTTACCCTACTCAACGCTAA