Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4015235..4015811 Replicon chromosome
Accession NZ_CP082327
Organism Escherichia coli strain B2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag K6983_RS19575 Protein ID WP_001297643.1
Coordinates 4015524..4015811 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag K6983_RS19570 Protein ID WP_000063156.1
Coordinates 4015235..4015537 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6983_RS19555 (4011831) 4011831..4013981 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
K6983_RS19560 (4014031) 4014031..4014234 + 204 WP_000467859.1 YbdD/YjiX family protein -
K6983_RS19565 (4014245) 4014245..4015201 + 957 WP_001297640.1 GTPase -
K6983_RS19570 (4015235) 4015235..4015537 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
K6983_RS19575 (4015524) 4015524..4015811 - 288 WP_001297643.1 BrnT family toxin Toxin
K6983_RS19580 (4016010) 4016010..4016707 - 698 Protein_3830 IS1 family transposase -
K6983_RS19585 (4016764) 4016764..4016859 + 96 Protein_3831 dienelactone hydrolase family protein -
K6983_RS19590 (4017005) 4017005..4018237 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
K6983_RS19595 (4018278) 4018278..4019558 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T214581 WP_001297643.1 NZ_CP082327:c4015811-4015524 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T214581 NZ_CP110145:3544035-3544253 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT214581 WP_000063156.1 NZ_CP082327:c4015537-4015235 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT214581 NZ_CP110145:3543634-3544008 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References