Detailed information of TA system TA214578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2383610..2384248
• Replicon: chromosome
• Accession: NZ_CP082327
• Organism: Escherichia coli strain B2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K6983_RS11555
• Protein ID: WP_000813794.1
• Coordinates: 2384072..2384248 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K6983_RS11550
• Protein ID: WP_001270286.1
• Coordinates: 2383610..2384026 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6983_RS11530 (2378762)	2378762..2379703	-	942	WP_001251313.1	ABC transporter permease	-
K6983_RS11535 (2379704)	2379704..2380717	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
K6983_RS11540 (2380735)	2380735..2381880	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K6983_RS11545 (2382125)	2382125..2383531	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
K6983_RS11550 (2383610)	2383610..2384026	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K6983_RS11555 (2384072)	2384072..2384248	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K6983_RS11560 (2384470)	2384470..2384700	+	231	WP_000494244.1	YncJ family protein	-
K6983_RS11565 (2384792)	2384792..2386753	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K6983_RS11570 (2386826)	2386826..2387362	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
K6983_RS11575 (2387454)	2387454..2388629	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2388669..2389934	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T214578 WP_000813794.1 NZ_CP082327:c2384248-2384072 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T214578 NZ_CP110145:1031642-1032124 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT214578 WP_001270286.1 NZ_CP082327:c2384026-2383610 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT214578 NZ_CP110145:1031388-1031654 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 6HPB