Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 824133..824787 Replicon chromosome
Accession NZ_CP082327
Organism Escherichia coli strain B2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K6983_RS04050 Protein ID WP_000244777.1
Coordinates 824380..824787 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K6983_RS04045 Protein ID WP_000354046.1
Coordinates 824133..824399 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6983_RS04020 (819302) 819302..820045 + 744 WP_000951964.1 SDR family oxidoreductase -
K6983_RS04025 (820102) 820102..821535 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K6983_RS04030 (821580) 821580..821891 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K6983_RS04035 (822055) 822055..822714 + 660 WP_000250274.1 hemolysin III family protein -
K6983_RS04040 (822910) 822910..823890 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K6983_RS04045 (824133) 824133..824399 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K6983_RS04050 (824380) 824380..824787 + 408 WP_000244777.1 protein YgfX Toxin
K6983_RS04055 (824827) 824827..825348 - 522 WP_001055874.1 flavodoxin FldB -
K6983_RS04060 (825460) 825460..826356 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K6983_RS04065 (826381) 826381..827091 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K6983_RS04070 (827097) 827097..828830 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T214567 WP_000244777.1 NZ_CP082327:824380-824787 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214567 NZ_CP110142:c4902957-4902673 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214567 WP_000354046.1 NZ_CP082327:824133-824399 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214567 NZ_CP110142:c4903189-4902947 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References