Detailed information of TA system TA214546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1336651..1337198
• Replicon: chromosome
• Accession: NZ_CP082313
• Organism: Vibrio alginolyticus strain ZZV2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6750_RS06425
• Protein ID: WP_238966996.1
• Coordinates: 1336896..1337198 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3A269
• Locus tag: K6750_RS06420
• Protein ID: WP_005448240.1
• Coordinates: 1336651..1336908 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6750_RS06355 (K6750_06350)	1331966..1332055	+	90	WP_079765356.1	DUF3265 domain-containing protein	-
K6750_RS06360 (K6750_06355)	1332474..1332602	+	129	WP_099098883.1	DUF3265 domain-containing protein	-
K6750_RS06365 (K6750_06360)	1333021..1333149	+	129	WP_099098883.1	DUF3265 domain-containing protein	-
K6750_RS06370 (K6750_06365)	1333177..1333728	+	552	WP_238966993.1	hypothetical protein	-
K6750_RS06375 (K6750_06370)	1333756..1333848	+	93	WP_140059861.1	DUF3265 domain-containing protein	-
K6750_RS06380 (K6750_06375)	1334095..1334562	+	468	WP_238966994.1	hypothetical protein	-
K6750_RS06385 (K6750_06380)	1334577..1334669	+	93	WP_004402681.1	DUF3265 domain-containing protein	-
K6750_RS06390 (K6750_06385)	1334698..1335192	+	495	WP_005536202.1	GNAT family N-acetyltransferase	-
K6750_RS06395 (K6750_06390)	1335183..1335299	+	117	WP_080731355.1	DUF3265 domain-containing protein	-
K6750_RS06400 (K6750_06395)	1335325..1335834	+	510	WP_213908296.1	DUF4303 domain-containing protein	-
K6750_RS06405 (K6750_06400)	1335857..1335949	+	93	WP_238966995.1	DUF3265 domain-containing protein	-
K6750_RS06410	1336438..1336515	+	78	Protein_1184	DUF3265 domain-containing protein	-
K6750_RS06420 (K6750_06410)	1336651..1336908	+	258	WP_005448240.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K6750_RS06425 (K6750_06415)	1336896..1337198	+	303	WP_238966996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6750_RS06430 (K6750_06420)	1337355..1337753	+	399	WP_238966997.1	DUF6232 family protein	-
K6750_RS06435 (K6750_06425)	1337768..1337860	+	93	WP_017821407.1	DUF3265 domain-containing protein	-
K6750_RS06440 (K6750_06430)	1337963..1338796	+	834	WP_238966998.1	hypothetical protein	-
K6750_RS06445 (K6750_06435)	1338949..1339581	+	633	WP_238966999.1	hypothetical protein	-
K6750_RS06450 (K6750_06440)	1339596..1339688	+	93	WP_238967000.1	DUF3265 domain-containing protein	-
K6750_RS06455 (K6750_06445)	1339717..1341234	+	1518	WP_238967001.1	SIR2 family protein	-
K6750_RS06460 (K6750_06450)	1341249..1341341	+	93	WP_072612521.1	DUF3265 domain-containing protein	-
K6750_RS06465 (K6750_06455)	1341374..1341958	+	585	WP_238951996.1	hypothetical protein	-
K6750_RS06470 (K6750_06460)	1341973..1342065	+	93	WP_077681052.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1320173..1343623	23450
	-	inside	Integrative and Conjugative Element	-	vxsC / exsA / exsD / vscB / vscC / vscD / vscF / vscG / vscH / vscI / vscJ / vscK / vscL / vopS / vopR / vecA / vopQ / vscU / vscT / vscS / vscR / vscQ / vscO / vscN / vopN / tyeA/vcr1 / sycN/vcr2 / vscX / vscY / vcrD / vcrR / vcrG / vcrV / vcrH / vopB / vopD / VP1611	1310270..1793048	482778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11624.53 Da
• Isoelectric Point: 5.8692

>T214546 WP_238966996.1 NZ_CP082313:1336896-1337198 [Vibrio alginolyticus]
MAEVIWTEPALSDLNDIAEYIALENIVAAKQLVQTIFSKVERLQTFPESGRIPPELEHLSYREVVVNPCRVFYKQDGNKV
FILFVMRAERDLRKFLLGKQ

Nucleotide

• Length: 303 bp

>T214546 NZ_CP110131:c5131381-5130884 [Methylorubrum extorquens]
GTGAGCCAGTCGCTCTGGCGGATCGGGACCGACGCTCGGACATACACTGCCGACGACCTGTCGGGGACCGGGGCCAAGCT
CTCCGGCGGCCGATGGAACGCCGTCGGCGTCGCCCTGGTCTACGCGGCGGGCAGTCGTGCCCTCGCCTGCCTCGAAACCT
TCGTCCACCTCAATGCCGGCGGCCTGCCGTTGAACCGATACCTCGTCGAAATCGACGTGCCGGATGAGGTCTGGGCGTCG
GCCGAACGCGCGACCCCGGACACGCTGCCCGTTGGCTGGGACGCCGAGCCGGCGGGGCTCGTCAGCCTCGCCTTCGGCAC
GGATTGGGCGAACGGAAATCGGTCGGCGCTTCTGCTGGTCCCCTCCGTCATCGTGCCGGAGGAGCAGAATGCCCTCATCA
ATCCCACCCATCCCGACGCGGCGAAAATCACGGCACGCAAGGTTCGCAAGTGGCTCTACGATCCGCGCCTCGACCGCGAC
CGAGGTCCCTTCGCGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9606.10 Da
• Isoelectric Point: 6.7269

>AT214546 WP_005448240.1 NZ_CP082313:1336651-1336908 [Vibrio alginolyticus]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLVDVDDYEFMQNRLAILEGIARGERALADGKVVSHDEAKDKM
SKWLK

Nucleotide

• Length: 258 bp

>AT214546 NZ_CP110131:c5131869-5131378 [Methylorubrum extorquens]
ATGCCGGACACGATGATCAAGGCCCCGGCCAAGCGGAGAAAGCCGCACGGGCCGGGGGCACGACCCTTCGCCTATCTCGA
CCTCTACCGCGCCTCCCCCTTCGACCGCATCGAGGTCATCAAGGCGGGGGTGCCCGCAAGGGCGGTCAAGGGTTTCATCA
CGGCGCTGTGCCTCGACCAGCAGGTGATGTTCGACGCCCTCAACCTGAAGACGGCCACGGTGAACAAGAAGGCGGCCCGG
GACGAAGCACTGTCGTCCGAGGACAGCGAGCGGGTCGTCGGCCTCGCCAAGCTCGTCGGGCAGCTCGAAGCGATGATCGA
GGAATCCGGCGCGACGAAGGATTTCGACGCGCCGGGATGGCTCTCGCGTTGGCTGCGCGAGCCCTTGCCGGCGCTGGGCG
GCGGCAAGCCGCTCGACCTCCTCGACACGATGGAGGGCCAAGCTCTGGTGTCGCGCGCGCTGGCGCAGATCCAGAGCGGA
GCCTATGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A432DGX8