Detailed information of TA system TA214531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3006742..3007359
• Replicon: chromosome
• Accession: NZ_CP082292
• Organism: Pantoea agglomerans strain CQ10

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0LTU7
• Locus tag: K6R05_RS14210
• Protein ID: WP_003850458.1
• Coordinates: 3007144..3007359 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: K6R05_RS14205
• Protein ID: WP_161736034.1
• Coordinates: 3006742..3007119 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6R05_RS14175 (K6R05_14175)	3003064..3003318	+	255	WP_222924421.1	type B 50S ribosomal protein L31	-
K6R05_RS14180 (K6R05_14180)	3003330..3003470	+	141	WP_009091664.1	type B 50S ribosomal protein L36	-
K6R05_RS14185 (K6R05_14185)	3003516..3004394	-	879	WP_161731951.1	metal ABC transporter substrate-binding protein	-
K6R05_RS14190 (K6R05_14190)	3004410..3005249	-	840	WP_222924423.1	metal ABC transporter permease	-
K6R05_RS14195 (K6R05_14195)	3005246..3005914	-	669	WP_222924425.1	ABC transporter ATP-binding protein	-
K6R05_RS14200 (K6R05_14200)	3006241..3006594	+	354	WP_222924426.1	hypothetical protein	-
K6R05_RS14205 (K6R05_14205)	3006742..3007119	+	378	WP_161736034.1	Hha toxicity modulator TomB	Antitoxin
K6R05_RS14210 (K6R05_14210)	3007144..3007359	+	216	WP_003850458.1	HHA domain-containing protein	Toxin
K6R05_RS14220 (K6R05_14220)	3007782..3008102	+	321	WP_161736031.1	MGMT family protein	-
K6R05_RS14225 (K6R05_14225)	3008132..3008689	-	558	WP_033731772.1	YbaY family lipoprotein	-
K6R05_RS14230 (K6R05_14230)	3008880..3009743	+	864	WP_161736030.1	acyl-CoA thioesterase II	-
K6R05_RS14235 (K6R05_14235)	3009802..3011088	-	1287	WP_161736029.1	ammonium transporter AmtB	-
K6R05_RS14240 (K6R05_14240)	3011123..3011461	-	339	WP_003850469.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (12-68)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8510.90 Da
• Isoelectric Point: 9.4825

>T214531 WP_003850458.1 NZ_CP082292:3007144-3007359 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Nucleotide

• Length: 216 bp

>T214531 NZ_CP110128:5112532-5112825 [Pseudomonas sp. WJP1]
ATGAATTATCTTGTCCAGCAAACAGCGATTTTCTCCGCATGGCACACCTCCATCCGTGATTTGCGGGCCAGGATTGCCAT
TGCTCGTCGCATTGAACGCGCTTGCGCGGGAAATTTGGGCGATATCAAACCAGTAGGTGATGGCGTTTCCGAAATGCGCG
TGGATGTAGGTGCCGGATACCGGATTTACTTCACGATGCGCAAGACTGTCGTCATCGTCTTGCTTGCAGGGGGCGACAAG
TCATCACAAAACGCTGATATCAAACGTGCAAAAAAATTGGCAAAGGAGGTCTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14659.29 Da
• Isoelectric Point: 4.5014

>AT214531 WP_161736034.1 NZ_CP082292:3006742-3007119 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGINSPDLQRWQRSAKRLFNLFTEECAFLHQPSHSL

Nucleotide

• Length: 378 bp

>AT214531 NZ_CP110128:5112828-5113136 [Pseudomonas sp. WJP1]
ATGAGCCAGACTTTGACGACTTTCGATATGGCGGCTCTGCTCGACAGCGACGAGGCCATCAGCGAGTACCTATCCCAGGT
GCTGGCAGACGGTGACAATGAGGAGTTTCTTCGTGCCGTCGGGTACGTTGTAAAAGCCCGCGGCATGACACAGGTAGCAA
AAAATTCCGGAATGGGTCGCGAAAGCTTGTACAAGGCCTTCGCTCCTGGCGCAAAACCACGTTTCAATACAGTTTTGAAA
GTGATTCACGCACTGGGTATCGATCTATGTGCGCAACCGGGTCATGTCATCAATCACTCAAACTCTTGA

Structures

Toxin

• AlphaFold DB: A0A1I5AGC3