Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5077916..5078541 Replicon chromosome
Accession NZ_CP082290
Organism Klebsiella pneumoniae subsp. pneumoniae strain Kp6

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6T93_RS24810 Protein ID WP_002882817.1
Coordinates 5077916..5078299 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6T93_RS24815 Protein ID WP_004150355.1
Coordinates 5078299..5078541 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T93_RS24795 (K6T93_24795) 5075282..5076184 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6T93_RS24800 (K6T93_24800) 5076181..5076816 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6T93_RS24805 (K6T93_24805) 5076813..5077742 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6T93_RS24810 (K6T93_24810) 5077916..5078299 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6T93_RS24815 (K6T93_24815) 5078299..5078541 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6T93_RS24820 (K6T93_24820) 5078746..5079663 + 918 WP_032422045.1 alpha/beta hydrolase -
K6T93_RS24825 (K6T93_24825) 5079677..5080618 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6T93_RS24830 (K6T93_24830) 5080663..5081100 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6T93_RS24835 (K6T93_24835) 5081097..5081957 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6T93_RS24840 (K6T93_24840) 5081951..5082550 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214526 WP_002882817.1 NZ_CP082290:c5078299-5077916 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214526 NZ_CP110128:424775-425062 [Pseudomonas sp. WJP1]
ATGACCGAACAATTCACTCGCTGGGACTCTGCCGAGTACCTCAAGACCGAAGAGGACATGGCAAATTATCTGGACGCTTG
CATGGAGGAAGCGGGAGATGATCCAGCATTCATCGCCAAAGCTCTCGGGACCATTGCACGTGCTCGCGGCATGACCCAGG
TCGCGCGGGATGCGGGGCTGTCTCGGGAAAGTCTCTACCGCGCATTGTCAGGTGAAGGGAATCCGGAGTTCGGGACCATC
CTGAAGGTCGTTAAGGCGTTAGGCTTGAAATTACACGCCAGCACCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214526 WP_004150355.1 NZ_CP082290:c5078541-5078299 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214526 NZ_CP110128:424473-424778 [Pseudomonas sp. WJP1]
ATGACGGAGATTCTTCGAAGTTCGACATTCTCCGGCTGGCTATTGAAGCTGGCCGATAGCCGCGCCAGGATGCGTATTCA
GGTGCGAATTGACCGCATGGCCGATGGAAATTTTGGTGATGTCAAAGCCATCGGAGAAGGGTTGAGTGAAGCACGTATCG
ACTATGGTCCCGGTTATCGGGTCTATTTCATGCAGCAAGGCAGCCAGTTGGTCATCCTCCTTTGTGGTGGAGACAAAAGC
AGCCAAACCCGCGACATCAAACAAGCCAGGCTCATTGCGAAATCCTGGCAGGAGCAAAACCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References