Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4473980..4474790 Replicon chromosome
Accession NZ_CP082290
Organism Klebsiella pneumoniae subsp. pneumoniae strain Kp6

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K6T93_RS21915 Protein ID WP_004178461.1
Coordinates 4473980..4474513 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6T93_RS21920 Protein ID WP_002887278.1
Coordinates 4474524..4474790 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T93_RS21910 (K6T93_21910) 4472811..4473932 + 1122 WP_102051411.1 cupin domain-containing protein -
K6T93_RS21915 (K6T93_21915) 4473980..4474513 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K6T93_RS21920 (K6T93_21920) 4474524..4474790 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6T93_RS21925 (K6T93_21925) 4474893..4476326 - 1434 WP_102051412.1 Cu(+)/Ag(+) sensor histidine kinase -
K6T93_RS21930 (K6T93_21930) 4476316..4476999 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K6T93_RS21935 (K6T93_21935) 4477172..4478557 + 1386 WP_032412712.1 efflux transporter outer membrane subunit -
K6T93_RS21940 (K6T93_21940) 4478575..4478919 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T214523 WP_004178461.1 NZ_CP082290:c4474513-4473980 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T214523 NZ_CP110127:1350278-1350727 [Mycolicibacterium fortuitum]
ATGGCCAAATTGTCTGTCTCCGTCGAAGTTCCGTTGCCGCCGGAGAAGGCGTGGGAGTACGCCTCTGATCTGTCCCGGTA
CGACGAGTGGCTCAGCATCCACCGGGCCTGGCGGTCGAAGTTGCCCGAAACCCTGGAAAAGGGCACGGTGATCGACTCGA
TCGTCGAGGTCAAGGGCATGTTGAACCGCGTGAAGTGGACGCTGGTGAACTACAAGCCGCCGCAGTCGTTGACCCTCAAC
GGCGACGGTCGTGGCGGGGTGAAGGTCAAGCTGATCGGAAAGATTACGCCGGCCGCCGTTGACGGTGGAGACGGTGCGAA
GGTCTCTTTCGACGTGCATCTGGGTGGGCCCGCGCTGTTCGGGCCGATCGGCATGGTGGTCGCCGCCGCGCTCAAGGGTG
ATATCCAGCAGTCGCTGAACAAGTTCAAGGAGCTCTACGCGTCGTCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT214523 WP_002887278.1 NZ_CP082290:c4474790-4474524 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT214523 NZ_CP110127:1350095-1350274 [Mycolicibacterium fortuitum]
ATGGGATTCCTGGACAAGGCCAAGGACCTTCTGTCGCAGAACGCCGACAAGGTCGAGCAGGCCATCGAGAAGGCCGGCGA
CATCGTCGACGAGAAGACTCAGGGCAAGTACTCGGGCGTGGTCGACAAGGCACAGGAAGCGGCGAAGAACGCCATCAACA
AGGAAGAACCGCAGCAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References