Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3039072..3039726 Replicon chromosome
Accession NC_007779
Organism Escherichia coli str. K-12 substr. W3110

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag Y75_RS15095 Protein ID WP_000244777.1
Coordinates 3039072..3039479 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag Y75_RS15100 Protein ID WP_000354046.1
Coordinates 3039460..3039726 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y75_RS15075 (Y75_p2824) 3035029..3036762 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
Y75_RS15080 (Y75_p2825) 3036768..3037478 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
Y75_RS15085 (Y75_p2826) 3037503..3038399 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
Y75_RS15090 (Y75_p2827) 3038511..3039032 + 522 WP_001055874.1 flavodoxin FldB -
Y75_RS15095 (Y75_p2828) 3039072..3039479 - 408 WP_000244777.1 toxin CptA Toxin
Y75_RS15100 (Y75_p2829) 3039460..3039726 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
Y75_RS15105 (Y75_p2830) 3039969..3040949 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
Y75_RS15110 (Y75_p2831) 3041145..3041804 - 660 WP_000250274.1 hemolysin III family protein -
Y75_RS15115 (Y75_p2832) 3041968..3042279 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
Y75_RS15120 (Y75_p2833) 3042324..3043757 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
Y75_RS15125 (Y75_p2834) 3043814..3044557 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T21452 WP_000244777.1 NC_007779:c3039479-3039072 [Escherichia coli str. K-12 substr. W3110]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T21452 NC_007779:c3039479-3039072 [Escherichia coli str. K-12 substr. W3110]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT21452 WP_000354046.1 NC_007779:c3039726-3039460 [Escherichia coli str. K-12 substr. W3110]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT21452 NC_007779:c3039726-3039460 [Escherichia coli str. K-12 substr. W3110]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References