Detailed information of TA system TA214508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1254142..1255059
• Replicon: chromosome
• Accession: NZ_CP082283
• Organism: Bacillus amyloliquefaciens strain JP3144

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BAJP3144_RS06505
• Protein ID: WP_007407256.1
• Coordinates: 1254313..1255059 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BAJP3144_RS06500
• Protein ID: WP_003154807.1
• Coordinates: 1254142..1254312 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAJP3144_RS06465 (1249375)	1249375..1250997	+	1623	WP_072177386.1	pyocin knob domain-containing protein	-
BAJP3144_RS06470 (1251010)	1251010..1251381	+	372	WP_017417608.1	XkdW family protein	-
BAJP3144_RS06475 (1251386)	1251386..1251583	+	198	WP_007610833.1	XkdX family protein	-
BAJP3144_RS06480 (1251640)	1251640..1252401	+	762	WP_094295696.1	phage portal protein	-
BAJP3144_RS06485 (1252453)	1252453..1252716	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BAJP3144_RS06490 (1252730)	1252730..1252993	+	264	WP_003154813.1	phage holin	-
BAJP3144_RS06495 (1253007)	1253007..1253885	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BAJP3144_RS20690 (1253920)	1253920..1254045	-	126	WP_003154809.1	hypothetical protein	-
BAJP3144_RS06500 (1254142)	1254142..1254312	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BAJP3144_RS06505 (1254313)	1254313..1255059	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BAJP3144_RS06510 (1255164)	1255164..1256162	-	999	WP_003154805.1	inorganic phosphate transporter	-
BAJP3144_RS06515 (1256175)	1256175..1256792	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BAJP3144_RS06520 (1257078)	1257078..1258394	-	1317	WP_007610842.1	amino acid permease	-
BAJP3144_RS06525 (1258716)	1258716..1259666	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1221518..1313245	91727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T214508 WP_007407256.1 NZ_CP082283:c1255059-1254313 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T214508 NZ_CP110117:c4443173-4443000 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT214508 WP_003154807.1 NZ_CP082283:c1254312-1254142 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT214508 NZ_CP110117:c4443497-4443201 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14