Detailed information of TA system TA214507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 480113..480750
• Replicon: chromosome
• Accession: NZ_CP082283
• Organism: Bacillus amyloliquefaciens strain JP3144

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAJP3144_RS02410
• Protein ID: WP_003156187.1
• Coordinates: 480400..480750 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAJP3144_RS02405
• Protein ID: WP_003156188.1
• Coordinates: 480113..480394 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAJP3144_RS02385 (476478)	476478..477077	-	600	WP_053573675.1	rhomboid family intramembrane serine protease	-
BAJP3144_RS02390 (477170)	477170..477535	+	366	WP_014304402.1	holo-ACP synthase	-
BAJP3144_RS02395 (477700)	477700..478707	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BAJP3144_RS02400 (478824)	478824..479993	+	1170	WP_021495079.1	alanine racemase	-
BAJP3144_RS02405 (480113)	480113..480394	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAJP3144_RS02410 (480400)	480400..480750	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAJP3144_RS02415 (480868)	480868..481689	+	822	WP_003156182.1	STAS domain-containing protein	-
BAJP3144_RS02420 (481694)	481694..482059	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
BAJP3144_RS02425 (482062)	482062..482463	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAJP3144_RS02430 (482475)	482475..483482	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BAJP3144_RS02435 (483546)	483546..483875	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BAJP3144_RS02440 (483872)	483872..484354	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BAJP3144_RS02445 (484320)	484320..485108	+	789	WP_029974100.1	RNA polymerase sigma factor SigB	-
BAJP3144_RS02450 (485108)	485108..485710	+	603	WP_128496463.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T214507 WP_003156187.1 NZ_CP082283:480400-480750 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T214507 NZ_CP110117:c4341004-4340654 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT214507 WP_003156188.1 NZ_CP082283:480113-480394 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT214507 NZ_CP110117:c4341249-4340998 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5