Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2775771..2776438 Replicon chromosome
Accession NC_007779
Organism Escherichia coli str. K-12 substr. W3110

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag Y75_RS13840 Protein ID WP_001094400.1
Coordinates 2776109..2776438 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag Y75_RS13835 Protein ID WP_000072690.1
Coordinates 2775771..2776088 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y75_RS13815 2770823..2771832 - 1010 Protein_2638 arsenic transporter -
Y75_RS13820 (Y75_p2588) 2771974..2773677 + 1704 WP_000896263.1 hypothetical protein -
Y75_RS24050 2774249..2774472 + 224 Protein_2640 DUF905 family protein -
Y75_RS13825 (Y75_p2589) 2774575..2775033 + 459 WP_000211841.1 antirestriction protein -
Y75_RS13830 (Y75_p2590) 2775042..2775524 + 483 WP_001407480.1 RadC family protein -
Y75_RS24055 2775533..2775733 + 201 WP_001395452.1 DUF987 domain-containing protein -
Y75_RS13835 (Y75_p2592) 2775771..2776088 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
Y75_RS13840 (Y75_p2593) 2776109..2776438 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
Y75_RS13845 (Y75_p2594) 2776802..2781382 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T21450 WP_001094400.1 NC_007779:2776109-2776438 [Escherichia coli str. K-12 substr. W3110]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T21450 NC_007779:2776109-2776438 [Escherichia coli str. K-12 substr. W3110]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT21450 WP_000072690.1 NC_007779:2775771-2776088 [Escherichia coli str. K-12 substr. W3110]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT21450 NC_007779:2775771-2776088 [Escherichia coli str. K-12 substr. W3110]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References