Detailed information of TA system TA214496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1097130..1097750
• Replicon: chromosome
• Accession: NZ_CP082280
• Organism: Enterobacter cancerogenus strain HAEC1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2ZG55
• Locus tag: HU826_RS05235
• Protein ID: WP_006176938.1
• Coordinates: 1097130..1097348 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D2ZG56
• Locus tag: HU826_RS05240
• Protein ID: WP_006176939.1
• Coordinates: 1097376..1097750 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU826_RS05205 (HU826_05205)	1093145..1093405	+	261	WP_222892329.1	type B 50S ribosomal protein L31	-
HU826_RS05210 (HU826_05210)	1093408..1093548	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
HU826_RS05215 (HU826_05215)	1093545..1094255	-	711	WP_153681429.1	GNAT family protein	-
HU826_RS05220 (HU826_05220)	1094357..1095817	+	1461	WP_153681428.1	PLP-dependent aminotransferase family protein	-
HU826_RS05225 (HU826_05225)	1095768..1096256	-	489	WP_042320836.1	YlaC family protein	-
HU826_RS05230 (HU826_05230)	1096373..1096924	-	552	WP_153681427.1	maltose O-acetyltransferase	-
HU826_RS05235 (HU826_05235)	1097130..1097348	-	219	WP_006176938.1	HHA domain-containing protein	Toxin
HU826_RS05240 (HU826_05240)	1097376..1097750	-	375	WP_006176939.1	Hha toxicity modulator TomB	Antitoxin
HU826_RS05245 (HU826_05245)	1098261..1101407	-	3147	WP_006176940.1	multidrug efflux RND transporter permease subunit AcrB	-
HU826_RS05250 (HU826_05250)	1101430..1102623	-	1194	WP_006176941.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T214496 WP_006176938.1 NZ_CP082280:c1097348-1097130 [Enterobacter cancerogenus]
MSEKPLTKIDYLMRLRRCQSLDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T214496 NZ_CP110117:1909838-1910092 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14467.25 Da
• Isoelectric Point: 4.8886

>AT214496 WP_006176939.1 NZ_CP082280:c1097750-1097376 [Enterobacter cancerogenus]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT214496 NZ_CP110117:1909590-1909841 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: D2ZG55

Antitoxin

• AlphaFold DB: D2ZG56