Detailed information of TA system TA214495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51008..51669
• Replicon: chromosome
• Accession: NZ_CP082280
• Organism: Enterobacter cancerogenus strain HAEC1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D2ZIF7
• Locus tag: HU826_RS00270
• Protein ID: WP_006177650.1
• Coordinates: 51271..51669 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HU826_RS00265
• Protein ID: WP_153682200.1
• Coordinates: 51008..51274 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU826_RS00235 (HU826_00235)	46116..46430	-	315	WP_042321291.1	PTS sugar transporter subunit IIB	-
HU826_RS00240 (HU826_00240)	46722..47672	+	951	WP_153682201.1	LacI family DNA-binding transcriptional regulator	-
HU826_RS00245 (HU826_00245)	47767..48063	-	297	WP_088208952.1	YicS family protein	-
HU826_RS00250 (HU826_00250)	48246..48665	+	420	WP_141113751.1	GNAT family N-acetyltransferase	-
HU826_RS00255 (HU826_00255)	48662..49309	-	648	WP_102891885.1	TetR/AcrR family transcriptional regulator	-
HU826_RS00260 (HU826_00260)	49403..50896	+	1494	WP_137273023.1	MDR family MFS transporter	-
HU826_RS00265 (HU826_00265)	51008..51274	+	267	WP_153682200.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HU826_RS00270 (HU826_00270)	51271..51669	+	399	WP_006177650.1	type II toxin-antitoxin system VapC family toxin	Toxin
HU826_RS00275 (HU826_00275)	51804..52604	+	801	WP_222892287.1	lipoprotein NlpA	-
HU826_RS00280 (HU826_00280)	52610..53290	-	681	WP_058609927.1	EAL domain-containing protein	-
HU826_RS00285 (HU826_00285)	53366..53986	-	621	WP_042321296.1	LuxR C-terminal-related transcriptional regulator	-
HU826_RS00290 (HU826_00290)	54649..55194	+	546	WP_241493789.1	LuxR C-terminal-related transcriptional regulator	-
HU826_RS00295 (HU826_00295)	55235..56299	-	1065	WP_058609928.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14606.72 Da
• Isoelectric Point: 5.9718

>T214495 WP_006177650.1 NZ_CP082280:51271-51669 [Enterobacter cancerogenus]
MRHMLDTNIVSHLFKRHPGVINRMACLTPDDVCISSITEAELLYGADKKQSERLKKTIQAFLSTITVCDWDSEAAATYGA
LRAAMEKRGNVMGDLDQLIAAHAISQGTTIVTNDRAFLMVQELSVEDWTHAA

Nucleotide

• Length: 399 bp

>T214495 NZ_CP110117:1909590-1909841 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10149.42 Da
• Isoelectric Point: 10.2633

>AT214495 WP_153682200.1 NZ_CP082280:51008-51274 [Enterobacter cancerogenus]
MERKAKLFKRGRSQAVMLPAEFTFDAKSVYIRRDDDGNVVLSEKPASGRSWDNFFRLLENTSVPENFLSKEERNQSITRR
DPLNGVQG

Nucleotide

• Length: 267 bp

>AT214495 NZ_CP110117:1909838-1910092 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: D2ZIF7