214488

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 473941..474578 Replicon chromosome
Accession NZ_CP082279
Organism Bacillus amyloliquefaciens strain Bam1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag K7I17_RS02405 Protein ID WP_003156187.1
Coordinates 474228..474578 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag K7I17_RS02400 Protein ID WP_003156188.1
Coordinates 473941..474222 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7I17_RS02380 (470307) 470307..470906 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
K7I17_RS02385 (470999) 470999..471364 + 366 WP_013351093.1 holo-ACP synthase -
K7I17_RS02390 (471529) 471529..472536 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
K7I17_RS02395 (472653) 472653..473822 + 1170 WP_013351095.1 alanine racemase -
K7I17_RS02400 (473941) 473941..474222 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
K7I17_RS02405 (474228) 474228..474578 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K7I17_RS02410 (474699) 474699..475520 + 822 WP_013351096.1 STAS domain-containing protein -
K7I17_RS02415 (475525) 475525..475890 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
K7I17_RS02420 (475893) 475893..476294 + 402 WP_013351097.1 anti-sigma regulatory factor -
K7I17_RS02425 (476306) 476306..477313 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
K7I17_RS02430 (477377) 477377..477706 + 330 WP_014469786.1 anti-sigma factor antagonist -
K7I17_RS02435 (477703) 477703..478185 + 483 WP_003156173.1 anti-sigma B factor RsbW -
K7I17_RS02440 (478151) 478151..478939 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
K7I17_RS02445 (478939) 478939..479541 + 603 WP_014469787.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T214488 WP_003156187.1 NZ_CP082279:474228-474578 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T214488 NZ_CP110117:185915-186022 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT214488 WP_003156188.1 NZ_CP082279:473941-474222 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT214488 NZ_CP110117:c185867-185801 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References