Detailed information of TA system TA214487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1224113..1225029
• Replicon: chromosome
• Accession: NZ_CP082278
• Organism: Bacillus amyloliquefaciens strain 35M

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: K7B13_RS06225
• Protein ID: WP_065981257.1
• Coordinates: 1224283..1225029 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: K7B13_RS06220
• Protein ID: WP_003154807.1
• Coordinates: 1224113..1224283 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B13_RS06190 (K7B13_06190)	1220979..1221359	+	381	WP_065981254.1	XkdW family protein	-
K7B13_RS06195 (K7B13_06195)	1221364..1221561	+	198	WP_013351962.1	XkdX family protein	-
K7B13_RS06200 (K7B13_06200)	1221609..1222370	+	762	WP_212101067.1	phage portal protein	-
K7B13_RS06205 (K7B13_06205)	1222424..1222687	+	264	WP_013351964.1	hemolysin XhlA family protein	-
K7B13_RS06210 (K7B13_06210)	1222701..1222964	+	264	WP_013351965.1	phage holin	-
K7B13_RS06215 (K7B13_06215)	1222978..1223856	+	879	WP_212101071.1	N-acetylmuramoyl-L-alanine amidase	-
K7B13_RS20225	1223891..1224016	-	126	WP_003154809.1	hypothetical protein	-
K7B13_RS06220 (K7B13_06220)	1224113..1224283	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K7B13_RS06225 (K7B13_06225)	1224283..1225029	-	747	WP_065981257.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K7B13_RS06230 (K7B13_06230)	1225137..1226135	-	999	WP_065981258.1	inorganic phosphate transporter	-
K7B13_RS06235 (K7B13_06235)	1226148..1226765	-	618	WP_003154804.1	DUF47 domain-containing protein	-
K7B13_RS06240 (K7B13_06240)	1227050..1228366	-	1317	WP_065981259.1	amino acid permease	-
K7B13_RS06245 (K7B13_06245)	1228690..1229640	+	951	WP_115997110.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29041.53 Da
• Isoelectric Point: 4.6839

>T214487 WP_065981257.1 NZ_CP082278:c1225029-1224283 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQTRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T214487 NZ_CP110115:47109-47402 [Arthrobacter sp. YA7-1]
GTGACCGGCGCGGAATCCACCGGGCCATCGCCATGGAACGTTGAGGTCACCAGCCCGGCGCTGAGGGGCTTCCGGCGACT
GCCTGAGAAGGCCGCGGCAGCGATCGTCGAATTCGTGACGGGCGCACTGGCCAGGAACCCCCACCGGCTGAGCAAGCCCC
TCACGAACGGACTCCTCGGACTCCGCACCGCACGGCGCGGCGACTACAGGGTGCTGTTCACCCTGGACATCGAAGAACAC
ATCCTGTACGTCCACCGCATCGAACATCGCTCCGACGTCTACAAGCCGCGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT214487 WP_003154807.1 NZ_CP082278:c1224283-1224113 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT214487 NZ_CP110115:46837-47112 [Arthrobacter sp. YA7-1]
ATGAGAACGGTACCGCTCAGCGAAGCCAAGGACAAGCTGTCCGCCCTCGTGGAGGAAGCAGACACGACGCACGAGATCAT
CCAGATCACCCGGCACGGGCACCCGTCAGCGGTGCTGATGTCCGCCGATGACCTGGAATCCCTGCAGGAAACCATCCACT
GGCTCTCACAGCCCGGCATCCGCGAAGACCTCGACCAGGCGAGGCGCGACATTGTCGACGGTAAGACGACCAGTGGCGCA
GACCTCCGCCGTGAGTTCGGGCTGCCGCCGCGGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14