Detailed information of TA system TA214486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 508470..509107
• Replicon: chromosome
• Accession: NZ_CP082278
• Organism: Bacillus amyloliquefaciens strain 35M

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K7B13_RS02430
• Protein ID: WP_003156187.1
• Coordinates: 508757..509107 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: K7B13_RS02425
• Protein ID: WP_003156188.1
• Coordinates: 508470..508751 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B13_RS02405 (K7B13_02405)	504831..505430	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
K7B13_RS02410 (K7B13_02410)	505523..505888	+	366	WP_115998347.1	holo-ACP synthase	-
K7B13_RS20165	506053..507060	+	1008	WP_065980767.1	outer membrane lipoprotein carrier protein LolA	-
K7B13_RS02420 (K7B13_02420)	507182..508351	+	1170	WP_115998346.1	alanine racemase	-
K7B13_RS02425 (K7B13_02425)	508470..508751	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K7B13_RS02430 (K7B13_02430)	508757..509107	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K7B13_RS02435 (K7B13_02435)	509228..510049	+	822	WP_065980769.1	STAS domain-containing protein	-
K7B13_RS02440 (K7B13_02440)	510054..510419	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
K7B13_RS02445 (K7B13_02445)	510422..510823	+	402	WP_065980770.1	anti-sigma regulatory factor	-
K7B13_RS02450 (K7B13_02450)	510835..511842	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
K7B13_RS02455 (K7B13_02455)	511906..512235	+	330	WP_014469786.1	anti-sigma factor antagonist	-
K7B13_RS02460 (K7B13_02460)	512232..512714	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
K7B13_RS02465 (K7B13_02465)	512680..513468	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
K7B13_RS02470 (K7B13_02470)	513468..514070	+	603	WP_065980772.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T214486 WP_003156187.1 NZ_CP082278:508757-509107 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T214486 NZ_CP110114:c3109953-3109672 [Arthrobacter sp. YA7-1]
ATGATCAGGTCGTTCGGGAGCAAAGACACCGAACGGCTGTGGAGTCGTGAGCATGTGGCTTCGATGGATTCACGGATCCT
TCGGTCCGCCCTCCGCAAGCTTCGCCAGGTGGGGTCCGCGGAGTCGATCGAGGATCTCCGCGTTCCGCCGGGGAACCGGC
TCGAAGCACTGAAAGGTGACCGGGCCGGTCCATACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCAGGTGGACA
GACGCTGGACCAGAGGAGGTGGAGATCGTTGACTACCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT214486 WP_003156188.1 NZ_CP082278:508470-508751 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT214486 NZ_CP110114:c3109685-3109383 [Arthrobacter sp. YA7-1]
TTGACTACCATTGACTATCTTGCGCCGATTCACCCAGGTGAAGTTCTCATGGAGGATTTCATTGTGGGTTTCGGCATCAC
GCAGAACAAGCTCGCCGTGGCGATCCATGTGCCGCCGCGCCGAATCAATGAGATAGTGCACGGCAAGCGTGCGATCACAG
CCGACACGGCACTACGTCTCGGCAAGTATTTTGGAACGTCGGCTCAGTTCTGGCTCAACCTGCAGACGCATTACGATCTC
GACGTTGCCGAAGATCGTGCGGCTGGGCAGATCGCGGCCATCACGCCTCTCAAGATCGCGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5