Detailed information of TA system TA214483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 1669779..1670380
• Replicon: chromosome
• Accession: NZ_CP082272
• Organism: Pasteurella multocida strain 17BRD-035

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K7B08_RS08285
• Protein ID: WP_223131907.1
• Coordinates: 1669779..1670093 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K7B08_RS08290
• Protein ID: WP_223131908.1
• Coordinates: 1670090..1670380 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B08_RS08235	1665511..1665672	-	162	WP_014390707.1	hypothetical protein	-
K7B08_RS08240	1665686..1665922	-	237	WP_016570068.1	hypothetical protein	-
K7B08_RS08245	1665909..1666193	-	285	WP_016570069.1	hypothetical protein	-
K7B08_RS08250	1666341..1666571	+	231	WP_223251317.1	hypothetical protein	-
K7B08_RS08255	1666633..1667268	-	636	WP_016569990.1	Bro-N domain-containing protein	-
K7B08_RS08260	1667645..1668055	+	411	WP_115173413.1	hypothetical protein	-
K7B08_RS08270	1668666..1668839	+	174	WP_167409471.1	hypothetical protein	-
K7B08_RS08275	1668826..1669065	-	240	WP_223131905.1	hypothetical protein	-
K7B08_RS08280	1669091..1669318	-	228	WP_223131906.1	hypothetical protein	-
K7B08_RS08285	1669779..1670093	+	315	WP_223131907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7B08_RS08290	1670090..1670380	+	291	WP_223131908.1	putative addiction module antidote protein	Antitoxin
K7B08_RS08295	1670423..1671055	-	633	WP_223131909.1	S24 family peptidase	-
K7B08_RS08300	1671193..1671399	+	207	WP_064965013.1	helix-turn-helix transcriptional regulator	-
K7B08_RS08305	1671448..1671897	+	450	WP_223131910.1	YmfL family putative regulatory protein	-
K7B08_RS08310	1671955..1672638	+	684	WP_223131911.1	phage antirepressor KilAC domain-containing protein	-
K7B08_RS13260	1672723..1673832	+	1110	WP_262495050.1	hypothetical protein	-
K7B08_RS08320	1673832..1675268	+	1437	WP_223131912.1	DnaB-like helicase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1657073..1751757	94684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11837.74 Da
• Isoelectric Point: 9.7597

>T214483 WP_223131907.1 NZ_CP082272:1669779-1670093 [Pasteurella multocida]
MFELIETTVFNKWLKELKDLSAKAAILARINRAKNGNFGDHKSVGDGLYEMRIMKGAGYRVYYTQYKDVTYLLICGGDKS
TQKADIAKAKVLWEEIKQKEGVNV

Nucleotide

• Length: 315 bp

>T214483 NZ_CP110109:3720759-3721109 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10560.20 Da
• Isoelectric Point: 5.7134

>AT214483 WP_223131908.1 NZ_CP082272:1670090-1670380 [Pasteurella multocida]
MSKKVELKPFDIAEHLDSEEMIAAYLSEVLETGDTNEFISALGDVARARGMTELAAKTGLGRESLYKTLSYGSKPRFDTI
MKITQALGIKLVPTHI

Nucleotide

• Length: 291 bp

>AT214483 NZ_CP110109:3720467-3720754 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA