Detailed information of TA system TA214480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 258990..259638
• Replicon: chromosome
• Accession: NZ_CP082270
• Organism: Stenotrophomonas sp. DFS-20110405

Toxin (Protein)

• Gene name: relE
• Uniprot ID: M5CT52
• Locus tag: K5L94_RS01205
• Protein ID: WP_004136948.1
• Coordinates: 258990..259277 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: M5D621
• Locus tag: K5L94_RS01210
• Protein ID: WP_004136950.1
• Coordinates: 259336..259638 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5L94_RS01175 (K5L94_01170)	254948..255739	-	792	WP_180878682.1	zinc-dependent peptidase	-
K5L94_RS01180 (K5L94_01175)	255706..255984	-	279	WP_008264959.1	hypothetical protein	-
K5L94_RS01185 (K5L94_01180)	256129..257097	+	969	WP_180878681.1	bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA	-
K5L94_RS01190 (K5L94_01185)	257094..257825	+	732	WP_274511761.1	type III pantothenate kinase	-
K5L94_RS01195 (K5L94_01190)	257835..258689	+	855	WP_274511764.1	SPOR domain-containing protein	-
K5L94_RS01205 (K5L94_01200)	258990..259277	+	288	WP_004136948.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5L94_RS01210 (K5L94_01205)	259336..259638	+	303	WP_004136950.1	putative addiction module antidote protein	Antitoxin
K5L94_RS01215 (K5L94_01210)	259811..260212	-	402	WP_208043275.1	hypothetical protein	-
K5L94_RS01220 (K5L94_01215)	260776..261720	+	945	WP_274511768.1	ankyrin repeat domain-containing protein	-
K5L94_RS01225 (K5L94_01220)	261825..262745	+	921	WP_274511771.1	arginase	-
K5L94_RS01230 (K5L94_01225)	262908..263045	+	138	WP_008266021.1	entericidin A/B family lipoprotein	-
K5L94_RS01235 (K5L94_01230)	263251..263472	+	222	WP_004153772.1	CsbD family protein	-
K5L94_RS01240 (K5L94_01235)	263546..264340	-	795	WP_274511776.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-95)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10807.40 Da
• Isoelectric Point: 10.7813

>T214480 WP_004136948.1 NZ_CP082270:258990-259277 [Stenotrophomonas sp. DFS-20110405]
MKIQRTRQFATWIDALKDVTGRARILARIGRLSEGHLGDHRYLADGVSELRIDVGPGYRVYYTQRGRQWVILLAGGDKGS
QQRDIEKAKEIARAL

Nucleotide

• Length: 288 bp

>T214480 NZ_CP082270:258990-259277 [Stenotrophomonas sp. DFS-20110405]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGGGCGCGCTCGCATCCTGGC
CCGCATCGGTCGGCTCAGCGAAGGCCATCTGGGCGATCACCGCTATCTGGCCGATGGCGTTTCCGAACTGCGCATCGATG
TGGGACCGGGCTACCGCGTGTACTACACCCAGCGCGGCAGGCAGTGGGTGATTCTGCTGGCTGGCGGCGACAAAGGTTCA
CAGCAACGCGATATCGAGAAGGCCAAAGAGATTGCGCGTGCCCTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10706.32 Da
• Isoelectric Point: 8.0343

>AT214480 WP_004136950.1 NZ_CP082270:259336-259638 [Stenotrophomonas sp. DFS-20110405]
MAIKKKVELKAFDVAEHLRTPEEMAAYLDACIEESDGDSAFIAKALGDIARAQGMSKVARAAGLSRESLYRALSGERSPD
FATILKVTRALGVRLHASAA

Nucleotide

• Length: 303 bp

>AT214480 NZ_CP082270:259336-259638 [Stenotrophomonas sp. DFS-20110405]
ATGGCCATCAAGAAGAAAGTCGAACTCAAAGCATTTGATGTGGCCGAGCACCTTCGTACACCGGAGGAGATGGCGGCCTA
TCTCGATGCCTGCATCGAAGAAAGCGATGGCGATTCTGCGTTCATTGCCAAGGCACTTGGCGATATCGCGCGCGCCCAGG
GCATGAGCAAGGTTGCGCGTGCGGCAGGCCTGTCGCGTGAGAGTCTGTATCGCGCACTGTCGGGCGAGCGCAGCCCTGAT
TTTGCGACCATCCTCAAAGTGACCCGCGCCCTGGGTGTACGCCTGCATGCCAGCGCAGCGTAA

Structures

Toxin

• AlphaFold DB: M5CT52

Antitoxin

• AlphaFold DB: M5D621