Detailed information of TA system TA214463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 251970..252556
• Replicon: chromosome
• Accession: NZ_CP082269
• Organism: Enterobacter sp. C2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: K4042_RS01165
• Protein ID: WP_222889335.1
• Coordinates: 252188..252556 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: K4042_RS01160
• Protein ID: WP_222889334.1
• Coordinates: 251970..252191 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4042_RS01135	247200..247586	-	387	WP_144818001.1	aspartate 1-decarboxylase autocleavage activator PanM	-
K4042_RS01140	248170..249096	+	927	WP_042393203.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
K4042_RS01145	249093..250370	+	1278	WP_144818003.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
K4042_RS01150	250367..251134	+	768	WP_103820364.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
K4042_RS01155	251152..251865	+	714	WP_042393287.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
K4042_RS01160	251970..252191	+	222	WP_222889334.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K4042_RS01165	252188..252556	+	369	WP_222889335.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K4042_RS01170	252723..254039	+	1317	WP_222889336.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
K4042_RS01175	254111..254998	+	888	WP_144818005.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
K4042_RS01180	254995..255840	+	846	WP_222889337.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
K4042_RS01185	255842..256915	+	1074	WP_222889338.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	250367..260411	10044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-117)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-55)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13428.62 Da
• Isoelectric Point: 7.4014

>T214463 WP_222889335.1 NZ_CP082269:252188-252556 [Enterobacter sp. C2]
MTLQFISAEEIIRFHDKLLSVTPGVAGMPDPGRAEALLYRVLNKHEYEGISDIWTLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLPANANYVDLTVAAAAGQLSLEEIALRLRG

Nucleotide

• Length: 369 bp

>T214463 NZ_CP110087:c1455369-1455112 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTAATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8050.09 Da
• Isoelectric Point: 6.4613

>AT214463 WP_222889334.1 NZ_CP082269:251970-252191 [Enterobacter sp. C2]
MRTMSYSEVRQNLAAALDSAAAGTPVTITRRGHKPAVIISTEEFERYQAAKLDAEFNAIMSVHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT214463 NZ_CP110087:c1455601-1455359 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCCTCTGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG