Detailed information of TA system TA214458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/-
• Location: 1092122..1092822
• Replicon: chromosome
• Accession: NZ_CP082267
• Organism: Enterococcus lactis strain E843

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: K4199_RS06285
• Protein ID: WP_252702561.1
• Coordinates: 1092122..1092541 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: K4199_RS06290
• Protein ID: WP_002313665.1
• Coordinates: 1092565..1092822 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4199_RS06260 (K4199_06255)	1087926..1088294	-	369	WP_002308916.1	DUF1801 domain-containing protein	-
K4199_RS06265 (K4199_06260)	1088444..1088938	+	495	WP_002316340.1	YbaK/EbsC family protein	-
K4199_RS06270 (K4199_06265)	1088962..1089972	+	1011	WP_002308918.1	LD-carboxypeptidase	-
K4199_RS06275 (K4199_06270)	1090088..1090798	-	711	WP_002308919.1	hypothetical protein	-
K4199_RS14380	1091062..1091379	+	318	Protein_1055	PTS alpha-glucoside transporter subunit IIBC	-
K4199_RS06280 (K4199_06275)	1091296..1091778	-	483	WP_146094118.1	DNA cytosine methyltransferase	-
K4199_RS06285 (K4199_06280)	1092122..1092541	-	420	WP_252702561.1	toxin	Toxin
K4199_RS06290 (K4199_06285)	1092565..1092822	-	258	WP_002313665.1	hypothetical protein	Antitoxin
K4199_RS14385	1092997..1093662	+	666	WP_002292140.1	hypothetical protein	-
K4199_RS06305 (K4199_06300)	1094153..1094383	+	231	WP_002329324.1	DUF6290 family protein	-
K4199_RS06310 (K4199_06305)	1094380..1094646	+	267	WP_070872447.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K4199_RS06315 (K4199_06310)	1094899..1095126	+	228	WP_070872449.1	hypothetical protein	-
K4199_RS06320 (K4199_06315)	1095142..1096308	+	1167	WP_070872451.1	radical SAM family heme chaperone HemW	-
K4199_RS06325 (K4199_06320)	1096542..1097585	+	1044	WP_104760045.1	heat-inducible transcriptional repressor HrcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16430.49 Da
• Isoelectric Point: 4.6731

>T214458 WP_252702561.1 NZ_CP082267:c1092541-1092122 [Enterococcus lactis]
VISEGLYFEAADLANELSEEVSYYCSKKMEHVKCYDIENYVNEVEDVEFIDYDFKDNLSKKMLGSITKVHGEIIITTNQH
LMLERKNFTKMHEIIHYYKDVPYLGESHAFSDMILEDAYLPEDYKKTSSKYRCQYPHGE

Nucleotide

• Length: 420 bp

>T214458 NZ_CP110081:c25890-25369 [Buttiauxella sp. WJP83]
GTGGCCGACTTGACCATAGAGATTTTCTCAGAAGGCAACGAATATGATTTTTCGGACTTCGATTGCGGTGAAGAGTCTCT
GAATGTTTTTCTGACAGATCATCTTGCTCGCCAGCATAGTGGTCGTATTTTACGTGGGTATTTGCTTTTAACCAAAGACC
CAAAACCTAAAGTCTTAGGGTATTACACACTGTCAGGTAGCTGTTTCGAAAAGGCCACTCTGCCCTCGAAAACACAGCAG
CGGAAAGTGCCTTATTCAAATGTGCCAAGTGTGACGCTCGGCCGCCTGGCTGTTCATAAAGACCTTCAGGGTAAAGAGTG
GGGAACGACACTCGTTACACACGCCATGAAGGTTGTATACAGCGCTTCCTTGGCAGTTGGTGTGCATGGGATGTTTGTTG
ATGCCCTAAATGATGGGGCAAAACGATTCTATACAAAGCTCGGCTTCATACCTTTGGTCGGCGATAACACAAACTCGCTT
TTCTATCCGACAAAGTCCATCGAGAAGCTGTTTGAAGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9913.09 Da
• Isoelectric Point: 4.2290

>AT214458 WP_002313665.1 NZ_CP082267:c1092822-1092565 [Enterococcus lactis]
MLSNYQLVTVIADFFNVSTDFLLNRTSDPTPPKRSEEDQLGDSILSHFRLNTADMEVEDIEELEEELNEYMDFLVQRAKA
KKNKK

Nucleotide

• Length: 258 bp

>AT214458 NZ_CP110081:c26163-25894 [Buttiauxella sp. WJP83]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGACTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GTCCAACCAGACTATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTTATTGAGCAACATCGCCGCC
TGATTCTCAACGAACAATCATGGAATCTGGTGATGGATGCCATCAGCAATCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAGCGTCTACAAAACATGGAGTAA