Detailed information of TA system TA214457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 157649..158757
• Replicon: plasmid pE843-171
• Accession: NZ_CP082266
• Organism: Enterococcus lactis strain E843

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: K4199_RS00890
• Protein ID: WP_000233000.1
• Coordinates: 157888..158757 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: K4199_RS00885
• Protein ID: WP_000205227.1
• Coordinates: 157649..157873 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4199_RS00870 (K4199_00870)	153279..154763	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
K4199_RS00875 (K4199_00875)	154817..155620	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
K4199_RS00880 (K4199_00880)	155929..157211	-	1283	Protein_175	ISL3 family transposase	-
K4199_RS00885 (K4199_00885)	157649..157873	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
K4199_RS00890 (K4199_00890)	157888..158757	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
K4199_RS00895 (K4199_00895)	158738..159472	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
K4199_RS00900 (K4199_00900)	159505..160413	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
K4199_RS00905 (K4199_00905)	160410..160952	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
K4199_RS00910 (K4199_00910)	161045..161839	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
K4199_RS00920 (K4199_00920)	162381..162476	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
K4199_RS00925 (K4199_00925)	162601..163338	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T214457 WP_000233000.1 NZ_CP082266:157888-158757 [Enterococcus lactis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T214457 NZ_CP110080:4454137-4454487 [Buttiauxella sp. WJP83]
ATGTGGGAAGTCATTACTACGGATGTTTTTGATCGCTGGTTTTTAGCGCAAGCCAATGCGCTAAGAGAAGATGTACTGGC
GGTAATGGGTATTCTGGAAGAAATGGGGCCACAACTTGGCCGTCCCTATGTGGATACACTCAATGGTTCCGCATTCCCTA
ATATGAAAGAACTTCGCATCCAGCATGCGGGGGTTCCAGTAAGAGCATTTTTCGCCTTTGATCCTGTCCGCCGAGCCATT
GTCTTATGCGCCGGTGATAAAACAGGGCTTAACGAGAAACAATTTTATCGCGACATGATTCGGCTGGCGGAATCTGAATA
TTGTAAGCACTTGGCCCATCTGGAGAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT214457 WP_000205227.1 NZ_CP082266:157649-157873 [Enterococcus lactis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT214457 NZ_CP110080:4454489-4454788 [Buttiauxella sp. WJP83]
ATGTCTACTTATAAAAAGTTACTCGCCAAACAGAGCCCGGAAAGCCAGCAGGAAATTGCGGATAAGATTCAGGTTTTACG
TCAGGAAATTGCTCTTAGCCAGCTTCGAGAGGAACTGAAGATGTCACAGACTGAACTCGCCCAGGCTGTTGGGGTATCCC
AACCTACCATAGCTAAAATGGAAAACCCTGAAAACGACCCGCGCTTATCCACATTAAAACGCTACGTCGCAGCTCTTGGG
GGTGAATTGAGTATTGATATAACGCTACCAACCGGTAAGCGTGTGGTTTTCCATCTCTGA

Structures

Toxin

• AlphaFold DB: R3JIN1