Detailed information of TA system TA214456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1308973..1309890
• Replicon: chromosome
• Accession: NZ_CP082262
• Organism: Bacillus velezensis strain BIM B-454D

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: K4A81_RS06825
• Protein ID: WP_007407256.1
• Coordinates: 1309144..1309890 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: K4A81_RS06820
• Protein ID: WP_003154807.1
• Coordinates: 1308973..1309143 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4A81_RS06785 (1304185)	1304185..1305774	+	1590	WP_103526959.1	hypothetical protein	-
K4A81_RS06790 (1305787)	1305787..1306212	+	426	WP_021493823.1	hypothetical protein	-
K4A81_RS06795 (1306217)	1306217..1306414	+	198	WP_007610833.1	XkdX family protein	-
K4A81_RS06800 (1306471)	1306471..1307232	+	762	WP_094295696.1	phage portal protein	-
K4A81_RS06805 (1307284)	1307284..1307547	+	264	WP_021493821.1	hemolysin XhlA family protein	-
K4A81_RS06810 (1307561)	1307561..1307824	+	264	WP_057079949.1	phage holin	-
K4A81_RS06815 (1307838)	1307838..1308716	+	879	WP_103526958.1	N-acetylmuramoyl-L-alanine amidase	-
K4A81_RS21145 (1308751)	1308751..1308876	-	126	WP_003154809.1	hypothetical protein	-
K4A81_RS06820 (1308973)	1308973..1309143	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K4A81_RS06825 (1309144)	1309144..1309890	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K4A81_RS06830 (1309994)	1309994..1310992	-	999	WP_003154805.1	inorganic phosphate transporter	-
K4A81_RS06835 (1311005)	1311005..1311622	-	618	WP_003154804.1	DUF47 domain-containing protein	-
K4A81_RS06840 (1311908)	1311908..1313224	-	1317	WP_007610842.1	amino acid permease	-
K4A81_RS06845 (1313546)	1313546..1314496	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T214456 WP_007407256.1 NZ_CP082262:c1309890-1309144 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T214456 NZ_CP110080:3605981-3606199 [Buttiauxella sp. WJP83]
ATGACAACTAAACCATTAACTAAGACCGATTATTTGATGCGCCTGCGACGCTGCCAAACAATTGATACCTTAGAACGTGT
AATTGAAAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGACTCGCTGAAC
TCACTATGAATAAGCTTTACGATAAAATACCACCTTCTGTATGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT214456 WP_003154807.1 NZ_CP082262:c1309143-1308973 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT214456 NZ_CP110080:3605577-3605951 [Buttiauxella sp. WJP83]
ATGGACGAATACTCACCAAAACGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAAAACCTGTATCATGACTGCCTTAC
CAACCTTGGCGAGAGTAATCATGGTTGGGTTAATGATCCTACTTCGGCCATTAACTTGCAGTTGAATGAATTGATTGAGC
ACATAGCTGCCTTCGCACTTAATTACAAAATTAAGTACGATGATGAGAGTAAATTGATTGAACAAATTGACGAATATTTG
GACGATACATTTATGCTATTCAGCAACTACGGCATCAACGCACAAGACTTGCAGAAATGGCGTAAGTCAGGGAATCGATT
ATTCCGATGTTTCGTCACAGAGAGCCGCGCTAACCCTGTAAGTCTCTCCTTTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14