Detailed information of TA system TA214455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 490589..491226
• Replicon: chromosome
• Accession: NZ_CP082262
• Organism: Bacillus velezensis strain BIM B-454D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K4A81_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 490876..491226 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: K4A81_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 490589..490870 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4A81_RS02460 (486954)	486954..487553	-	600	WP_128496462.1	rhomboid family intramembrane serine protease	-
K4A81_RS02465 (487646)	487646..488011	+	366	WP_014304402.1	holo-ACP synthase	-
K4A81_RS02470 (488176)	488176..489183	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
K4A81_RS02475 (489300)	489300..490469	+	1170	WP_021495079.1	alanine racemase	-
K4A81_RS02480 (490589)	490589..490870	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K4A81_RS02485 (490876)	490876..491226	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K4A81_RS02490 (491344)	491344..492165	+	822	WP_003156182.1	STAS domain-containing protein	-
K4A81_RS02495 (492170)	492170..492535	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
K4A81_RS02500 (492538)	492538..492939	+	402	WP_003156178.1	anti-sigma regulatory factor	-
K4A81_RS02505 (492951)	492951..493958	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
K4A81_RS02510 (494022)	494022..494351	+	330	WP_016937169.1	anti-sigma factor antagonist	-
K4A81_RS02515 (494348)	494348..494830	+	483	WP_103527127.1	anti-sigma B factor RsbW	-
K4A81_RS02520 (494796)	494796..495584	+	789	WP_029974100.1	RNA polymerase sigma factor SigB	-
K4A81_RS02525 (495584)	495584..496186	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T214455 WP_003156187.1 NZ_CP082262:490876-491226 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T214455 NZ_CP110080:c3525354-3524962 [Buttiauxella sp. WJP83]
ATGGCATACATGCTGGATACCAATACGGTGAGCTATTTTTTGCGCCAGCATCCGCAAATTGTTGCCAGGATATCCGCAGT
CCCTCCGTCGAGTATTTCGATTTCAAGTATTACCGAAGCGGAGCTGCTTTTTGGTGTGGAGAAACGGCGAAATAAAGCGC
TTAAAACCGCTGTAATGGGGTTTCTTGATGCAGTATCGATATATGAATGGGACAGTGCTGCTGCGCAAACCTACGGTAAA
TTAAGGGCGAGCATGGAAAAGAAAGGGCACGTTATGGGGGCTCTCGATATGCTTATCGCGGCCCATGCCCTAAGTGAAAA
AACAACTCTGATTACCAGCGATCGCGCATTCTATGGTGTCGCAGGGTTGCAGGTACAAGACTGGACGGTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT214455 WP_003156188.1 NZ_CP082262:490589-490870 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT214455 NZ_CP110080:c3525617-3525354 [Buttiauxella sp. WJP83]
ATGGAGCGCATTGCTAAGCTTTTTAAAAATGGCAGAAACCAGGCGGTGAGGTTACCGGTGGAATTTGAGTTCGATACGGA
CAGGGTATATATCCGACAAGACAAGGAGGGGAATGTGATTCTTTCAAAAAGGCCAGCAAAACCAGACAACTGGGATAACG
TGATTTCACTGATAAAAAAGGCCCGTGTTCCTGATAATTTCCTGGATGCAGAGGAACGCAATCAGCCTCCTGCTGACAGA
GATCCTTTTGCGGGAATTAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5