Detailed information of TA system TA214454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4705404..4706543
• Replicon: chromosome
• Accession: NZ_CP082244
• Organism: Pseudomonas otitidis strain CSMC7.1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K6751_RS21515
• Protein ID: WP_222902148.1
• Coordinates: 4705404..4706153 (-)
• Length: 250 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1E4WFB3
• Locus tag: K6751_RS21520
• Protein ID: WP_044405544.1
• Coordinates: 4706172..4706543 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6751_RS21505 (K6751_21505)	4703075..4704436	-	1362	WP_222902147.1	DNA recombination protein RmuC	-
K6751_RS21510 (K6751_21510)	4704603..4705016	-	414	WP_044405541.1	SEL1-like repeat protein	-
K6751_RS21515 (K6751_21515)	4705404..4706153	-	750	WP_222902148.1	RES family NAD+ phosphorylase	Toxin
K6751_RS21520 (K6751_21520)	4706172..4706543	-	372	WP_044405544.1	XRE family transcriptional regulator	Antitoxin
K6751_RS21525 (K6751_21525)	4706676..4707158	+	483	WP_044405545.1	GreA/GreB family elongation factor	-
K6751_RS21530 (K6751_21530)	4707195..4707590	+	396	WP_044405547.1	hypothetical protein	-
K6751_RS21535 (K6751_21535)	4707795..4707968	+	174	WP_167368480.1	hypothetical protein	-
K6751_RS21540 (K6751_21540)	4707996..4708421	-	426	WP_074971868.1	S24 family peptidase	-
K6751_RS21545 (K6751_21545)	4708531..4710135	-	1605	WP_222900040.1	M28 family metallopeptidase	-
K6751_RS21550 (K6751_21550)	4710411..4711076	-	666	WP_044405551.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (58-205)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 250 a.a.
• Molecular weight: 28159.08 Da
• Isoelectric Point: 6.9626

>T214454 WP_222902148.1 NZ_CP082244:c4706153-4705404 [Pseudomonas otitidis]
MDIWSACDGERHIVPLRGRVVRLVESQEQVATLQLVDNLAEQALLEELIETSKPPLPRSAEPLHYLLKTPFRYPPLRWGS
RFGTVHEPSLFYAAARLETAMAEGAFYRFVLWSGMATPPPSGRILSEHASFEARFQVERGVSLQNPPFDAYPALLDRRDY
RATQALGAAMRAAGVEAFEYRSARCPQQGLNIALFTPDAFTERRPRNLTPWFCETTADYVAYKHAQAPDQPRLFRREAFL
VDGVIPQPA

Nucleotide

• Length: 750 bp

>T214454 NZ_CP110080:2494396-2494671 [Buttiauxella sp. WJP83]
ATGGGATATGACAAGGATTTTCTCAGAAACAAACATCAGGGCACGTTAAAGCAGATTTTTGCCAAACCCACGTCGACCAG
CATCAGATGGCAGGATGTTGAAATGTTGATTATTGCACTAAACGGTGAAGTCAGAAGCGGTAAGGGATCGCGGGTGCGCT
TCCTGTTAAAAGGCAGTATTGCGCATTTTCACCGCCCCCATCCGTCACCCGAGACTGACAAAGGTGCCATCGTCAATTTG
CAAGAATGGTTTGAAAGTATTGGAGTAACACCATGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13690.98 Da
• Isoelectric Point: 10.6556

>AT214454 WP_044405544.1 NZ_CP082244:c4706543-4706172 [Pseudomonas otitidis]
MSALTRPHLDPDAVLAKALLNTREQLGLTRQQLADIVGVHRTVVTRWADAGSIRPQSKTGELALLVIRIYRALFALFGGN
LEDMRHFLRTENRHLGGVPLELMGQVQGLVRVVEYLDAIRGKV

Nucleotide

• Length: 372 bp

>AT214454 NZ_CP110080:2494683-2495030 [Buttiauxella sp. WJP83]
ATGATCCCCAACACCATGGTGATTTGTGGGCAACCCGCATTGATCACCTGGGTTCCAGAAATCGGCTTGTTTCGCGGTAA
ATTTCTTGGTCTTTCAGGCTACTGTGATTTTGTCGCGGATAGTGCGAAGGGATTGATGGCTGAAGGCGAACTTTCGTTAA
AAGAGTATCTGGATGATTGTAAAACCAGCGGCATTGATCCTTATGAGCGGGAAGAAAAAATAAAGACGTTCACCCTGCGT
TATCCGGAGTCTTTCGGTGAACGTCTGGCGATTGCCGCTGCTGAACAACGTATGTCAGTTAATAGCTATATCGTTGAAAT
GCTGAGTAAACAGATGAAATTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1E4WFB3