Detailed information of TA system TA214453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1719710..1721118
• Replicon: chromosome
• Accession: NZ_CP082244
• Organism: Pseudomonas otitidis strain CSMC7.1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K6751_RS08055
• Protein ID: WP_142011214.1
• Coordinates: 1719710..1720279 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A542V6R8
• Locus tag: K6751_RS08060
• Protein ID: WP_074968953.1
• Coordinates: 1720276..1721118 (+)
• Length: 281 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6751_RS08045 (K6751_08045)	1714712..1715911	-	1200	WP_182852135.1	GGDEF domain-containing protein	-
K6751_RS08050 (K6751_08050)	1715998..1719474	-	3477	WP_044406195.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
K6751_RS08055 (K6751_08055)	1719710..1720279	+	570	WP_142011214.1	GNAT family protein	Toxin
K6751_RS08060 (K6751_08060)	1720276..1721118	+	843	WP_074968953.1	AraC family transcriptional regulator	Antitoxin
K6751_RS08065 (K6751_08065)	1721156..1721869	+	714	WP_142011212.1	AzlC family ABC transporter permease	-
K6751_RS08070 (K6751_08070)	1721869..1722198	+	330	WP_074968949.1	AzlD domain-containing protein	-
K6751_RS08075 (K6751_08075)	1722328..1724202	+	1875	WP_222901399.1	MFS transporter	-
K6751_RS08080 (K6751_08080)	1724256..1724570	-	315	WP_044406178.1	quaternary ammonium compound efflux SMR transporter SugE	-
K6751_RS08085 (K6751_08085)	1724734..1725687	-	954	WP_044406176.1	bile acid:sodium symporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (18-149)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21147.17 Da
• Isoelectric Point: 7.4044

>T214453 WP_142011214.1 NZ_CP082244:1719710-1720279 [Pseudomonas otitidis]
MFNPQPVTLQRGALRLEPLGEADVSELAALAEANRNELVYMSGPLRPDWYRQGIAQQREGQAVVFAIRLGDRLVGTSRFA
EFMPALPAVEIGWTWLAGSEHGTGLNASIKYLMLRHAFEEWQVVRVQLKTAGSNLRSQRAIEKLGAVREGVLRNHRRLAD
GRLDDSVMFSITDHEWPAVKQALEAAFVQ

Nucleotide

• Length: 570 bp

>T214453 NZ_CP110080:1933234-1933338 [Buttiauxella sp. WJP83]
GGCAAGGCGAAGTCTGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATA
TAGGAATCGTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 281 a.a.
• Molecular weight: 31273.53 Da
• Isoelectric Point: 9.0216

>AT214453 WP_074968953.1 NZ_CP082244:1720276-1721118 [Pseudomonas otitidis]
MSTGGERTRFWQASALSGVELLHARYIQHRFSPHVHEGYVFTILESGAQRFRHRGSDHLAAVGSMVLINPDELHTGSKAH
EEGWRYRGFYPDLHRVTDVLDELEIATGGLPTFADSVLHDPEVTRRFLDLHCLLERNASALEQQVAWREAILLLFSRHAR
IGAPRPAGQEPQAVALAREVLAARLASPPSLEELAALVGLSPFHFARVFQRATGMPPHAWLKQRRLEQARAMLRSGCAPL
SVAMQLGFADQSHLTRQFKQTYGVGPGEYRTASARSFKTD

Nucleotide

• Length: 843 bp

>AT214453 NZ_CP110080:c1933344-1933199 [Buttiauxella sp. WJP83]
ACAGATAAAAAAAGACCGAATACGATTCCTATATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCAGACTTCGCCTTGCCTTCTAAGAATAGATTACCGGCGCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A542V6R8