Detailed information of TA system TA214450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2793386..2794022
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7J980
• Locus tag: K7B00_RS14325
• Protein ID: WP_012537455.1
• Coordinates: 2793386..2793802 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J981
• Locus tag: K7B00_RS14330
• Protein ID: WP_009560865.1
• Coordinates: 2793789..2794022 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS14295 (K7B00_14295)	2788725..2789012	-	288	WP_012537449.1	DDE-type integrase/transposase/recombinase	-
K7B00_RS14300 (K7B00_14300)	2789375..2790301	-	927	WP_012537450.1	uracil-DNA glycosylase	-
K7B00_RS14305 (K7B00_14305)	2790468..2791538	+	1071	WP_012537451.1	linear amide C-N hydrolase	-
K7B00_RS14310 (K7B00_14310)	2791751..2792116	-	366	WP_257245758.1	type II toxin-antitoxin system PemK/MazF family toxin	-
K7B00_RS14315 (K7B00_14315)	2792131..2792382	-	252	WP_012537453.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
K7B00_RS14320 (K7B00_14320)	2792702..2793283	+	582	WP_146235847.1	hypothetical protein	-
K7B00_RS14325 (K7B00_14325)	2793386..2793802	-	417	WP_012537455.1	PIN domain-containing protein	Toxin
K7B00_RS14330 (K7B00_14330)	2793789..2794022	-	234	WP_009560865.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K7B00_RS14335 (K7B00_14335)	2794021..2794281	+	261	WP_012607558.1	hypothetical protein	-
K7B00_RS14340 (K7B00_14340)	2794378..2794728	+	351	WP_012537457.1	DUF4870 domain-containing protein	-
K7B00_RS14345 (K7B00_14345)	2794825..2796687	+	1863	WP_012537458.1	ATP-binding cassette domain-containing protein	-
K7B00_RS14350 (K7B00_14350)	2796684..2797007	-	324	WP_012537459.1	hypothetical protein	-
K7B00_RS14355 (K7B00_14355)	2797000..2797311	-	312	WP_009566855.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15358.75 Da
• Isoelectric Point: 5.1352

>T214450 WP_012537455.1 NZ_CP082238:c2793802-2793386 [Acidithiobacillus ferrooxidans]
MASGKAFFDTNVLLYLLSADTAKADVAEGIMVNGGVISAQVLNEFASVAQRKMGMSFLEIREVLTPIHRICDVAHLTLDT
HIHGLRIAERYKYNVWDAMIVASALLADCAVLYTEDMHHGQILEDRLQLINPFLRADE

Nucleotide

• Length: 417 bp

>T214450 NZ_CP110080:277054-277320 [Buttiauxella sp. WJP83]
ATGAAGCCGAAAATTACGCAGGAAGCGGCCGCAGATATAGCCTGGTGGAAACAAAATAACCCAAAAGTAAAAGCTAAAAT
CGACGAACTCGTCAGAGCTGCTCTGGAAGACCCTAAATCTGGTATCGGTAAGCCAGAACCGTTGAAGCACCATAAGCCCT
TATGGTCGCGACGTATTACTCAGGAGCACCGTTTTGTCTATTACGTGGATGCGGAGAATGACCTTCTGATTATTGTCTCT
TGCCGCTACCACTACACAAATATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8919.15 Da
• Isoelectric Point: 8.7371

>AT214450 WP_009560865.1 NZ_CP082238:c2794022-2793789 [Acidithiobacillus ferrooxidans]
MQVSKWGNSLAVRIPTSVVEALGIKEGDDIEVEVVGERRFQIQRTPQAEELLARLRRYRGRLPADFRFDRLEAHGER

Nucleotide

• Length: 234 bp

>AT214450 NZ_CP110080:276809-277057 [Buttiauxella sp. WJP83]
ATGCAACACATTAATTATACCGATGCCCGCGCAAACCTGGCGGCGCTGATGCAACAGACCAATGACGACCGTGCACCGGT
GCTCATCACGCGCAAAGGCGCGGGTAGTGTTGTCGTCATTGATGCGGCTGAATTTGAGTCCATTCAAGAAACATTACATC
TGTTTAAAAATCCGGTGAATGCGGCGCATATCGAACGTTCGCTGGAACAGGCCGATCGTGCTGAACTGACGGATTATGAA
TTTGAATGA

Structures

Toxin

• AlphaFold DB: B7J980

Antitoxin

• AlphaFold DB: B7J981