Detailed information of TA system TA214449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2688316..2688960
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K7B00_RS13765
• Protein ID: WP_229129810.1
• Coordinates: 2688316..2688600 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J8J7
• Locus tag: K7B00_RS13770
• Protein ID: WP_009566530.1
• Coordinates: 2688721..2688960 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS13725 (K7B00_13725)	2683451..2684089	+	639	WP_225487201.1	lipoprotein insertase outer membrane protein LolB	-
K7B00_RS13730 (K7B00_13730)	2684086..2684931	+	846	WP_012537374.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
K7B00_RS13740 (K7B00_13740)	2685059..2686006	+	948	WP_009567132.1	ribose-phosphate pyrophosphokinase	-
K7B00_RS13750 (K7B00_13750)	2686359..2686721	-	363	Protein_2707	IS3 family transposase	-
K7B00_RS13755 (K7B00_13755)	2686840..2687751	-	912	WP_041646513.1	DUF2034 domain-containing protein	-
K7B00_RS13760 (K7B00_13760)	2688040..2688192	+	153	WP_009566528.1	hypothetical protein	-
K7B00_RS13765 (K7B00_13765)	2688316..2688600	-	285	WP_229129810.1	PIN domain-containing protein	Toxin
K7B00_RS13770 (K7B00_13770)	2688721..2688960	-	240	WP_009566530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K7B00_RS13775 (K7B00_13775)	2689141..2689410	-	270	WP_012537379.1	hypothetical protein	-
K7B00_RS13780 (K7B00_13780)	2689629..2690510	-	882	WP_012607524.1	glutathione-dependent disulfide-bond oxidoreductase	-
K7B00_RS13785 (K7B00_13785)	2691159..2691944	-	786	WP_012537380.1	sulfite exporter TauE/SafE family protein	-
K7B00_RS13790 (K7B00_13790)	2692010..2692345	-	336	WP_041838695.1	thiamine pyrophosphate-dependent enzyme	-
K7B00_RS13795 (K7B00_13795)	2692420..2693247	-	828	WP_012536793.1	IS21-like element ISAfe9 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2686359..2686739	380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-84)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10161.72 Da
• Isoelectric Point: 6.7118

>T214449 WP_229129810.1 NZ_CP082238:c2688600-2688316 [Acidithiobacillus ferrooxidans]
VVCELRYGAAKKNSPRLTEQVNAVIAAMDVLPYDTPVDQAYAEIRQHLAAYGQPIGPNDLLIASHALAVKAILVSANVGE
FSRVPGLIVHNWLQ

Nucleotide

• Length: 285 bp

>T214449 NZ_CP110080:c275976-275596 [Buttiauxella sp. WJP83]
ATGGTGGAATCATCGGCTATTTTTGATACCAACATCCTGATTGATTACTTAAATGGCGTTCCCGAAGCAAAGGTAGCCCT
GACTGCTTACGGATCTAAACCTGCGATAAGTGTGATCACATGGATAGAGGTCATGGTGGGGGCTAAACGATTCGGTCATG
AACAACAGACAAAACAATTTCTGAGCGGGTTTGATGTATTACCCGTAAATCAGGCAGTTTCAGAACAGGCTGTAGAAACT
CGTCAGGAATACGGCATGAAGTTGCCAGATGCCATCATTCTTGCGACGGCCAAAGTGAATCTGAAACGCCTAATCACCCG
CAATAGCAAAGACTTCAAAAACATTCCTGGTGTAGTGATGCCTTATTCACTGGCTTGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9405.66 Da
• Isoelectric Point: 4.7132

>AT214449 WP_009566530.1 NZ_CP082238:c2688960-2688721 [Acidithiobacillus ferrooxidans]
MHTERHVRFFRNGRNQAVRIPREFEMDAQEAIMRREDDRLIIEPVKRKGLLATLASLSALDEEFPDTDRDLLPLDDIEW

Nucleotide

• Length: 240 bp

>AT214449 NZ_CP110080:c276218-275976 [Buttiauxella sp. WJP83]
ATGGCAACATTGTTTGGAGAAATGATGGGACGAATTCTTCTGGATCTGTCAGACGAAGTAGTGCGCCGTCTGGACGATTT
AAAGCAAAGCCGTAATCTTCCTCGTGCAGAATTACTTCGAGAAGCCGTTGATGAGTATCTCGAAAGACATACGCAGGTTG
CCGTAACAGAGGCTTTTGGCCTTTGGAGTGAGCATGCCGTCGACGGTGTGGAATATGAACGCACCCTTCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: B7J8J7