Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 2293178..2293746 Replicon chromosome
Accession NZ_CP082238
Organism Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)


Gene name vapC Uniprot ID A0A5J6VSB9
Locus tag K7B00_RS11665 Protein ID WP_012536619.1
Coordinates 2293372..2293746 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2Z6IL32
Locus tag K7B00_RS11660 Protein ID WP_012536620.1
Coordinates 2293178..2293375 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7B00_RS11620 (K7B00_11620) 2288476..2289372 + 897 WP_012536626.1 hypothetical protein -
K7B00_RS11625 (K7B00_11625) 2289397..2289666 + 270 WP_012536625.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
K7B00_RS11630 (K7B00_11630) 2289653..2289787 + 135 Protein_2291 type II toxin-antitoxin system YafQ family toxin -
K7B00_RS16820 2289836..2289967 - 132 WP_012607115.1 hypothetical protein -
K7B00_RS16825 2290390..2290611 - 222 WP_009560828.1 HU family DNA-binding protein -
K7B00_RS11635 (K7B00_11635) 2290620..2290973 - 354 WP_151528214.1 hypothetical protein -
K7B00_RS11640 (K7B00_11640) 2290985..2291260 - 276 WP_012536624.1 HU family DNA-binding protein -
K7B00_RS11645 (K7B00_11645) 2291327..2291650 - 324 WP_180830446.1 integration host factor subunit alpha -
K7B00_RS11650 (K7B00_11650) 2291647..2292321 - 675 WP_012536622.1 hypothetical protein -
K7B00_RS11655 (K7B00_11655) 2292671..2292832 + 162 WP_012536621.1 hypothetical protein -
K7B00_RS11660 (K7B00_11660) 2293178..2293375 + 198 WP_012536620.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K7B00_RS11665 (K7B00_11665) 2293372..2293746 + 375 WP_012536619.1 type II toxin-antitoxin system VapC family toxin Toxin
K7B00_RS16830 2293671..2294093 + 423 WP_225981991.1 N-6 DNA methylase -
K7B00_RS11675 (K7B00_11675) 2294148..2296946 + 2799 WP_012536617.1 class I SAM-dependent DNA methyltransferase -
K7B00_RS11680 (K7B00_11680) 2296943..2297602 + 660 WP_012536616.1 putative DNA binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-109)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-46)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13683.71 Da        Isoelectric Point: 6.3157

>T214448 WP_012536619.1 NZ_CP082238:2293372-2293746 [Acidithiobacillus ferrooxidans]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTSSE

Download         Length: 375 bp

>T214448 NZ_CP110076:c54908-54567 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7266.37 Da        Isoelectric Point: 9.4896

>AT214448 WP_012536620.1 NZ_CP082238:2293178-2293375 [Acidithiobacillus ferrooxidans]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Download         Length: 198 bp

>AT214448 NZ_CP110076:c55138-54908 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5J6VSB9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z6IL32

References