Detailed information of TA system TA214447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2263436..2263885
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: K7B00_RS11480
• Protein ID: WP_012536654.1
• Coordinates: 2263739..2263885 (-)
• Length: 49 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K7B00_RS11475
• Protein ID: WP_012536655.1
• Coordinates: 2263436..2263735 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS11445 (K7B00_11445)	2259292..2259669	-	378	WP_012536661.1	VirB3 family type IV secretion system protein	-
K7B00_RS11450 (K7B00_11450)	2259673..2260059	-	387	WP_012536660.1	TrbC/VirB2 family protein	-
K7B00_RS11455 (K7B00_11455)	2260264..2261055	+	792	WP_012536659.1	hypothetical protein	-
K7B00_RS11460 (K7B00_11460)	2261452..2262420	-	969	WP_012536657.1	P-type conjugative transfer ATPase TrbB	-
K7B00_RS11465 (K7B00_11465)	2262417..2262869	-	453	WP_012536656.1	hypothetical protein	-
K7B00_RS11470 (K7B00_11470)	2263022..2263327	-	306	WP_146235838.1	hypothetical protein	-
K7B00_RS11475 (K7B00_11475)	2263436..2263735	-	300	WP_012536655.1	putative addiction module antidote protein	Antitoxin
K7B00_RS11480 (K7B00_11480)	2263739..2263885	-	147	WP_012536654.1	addiction module killer protein	Toxin
K7B00_RS11485 (K7B00_11485)	2264317..2264460	-	144	WP_012536652.1	hypothetical protein	-
K7B00_RS11490 (K7B00_11490)	2264568..2264900	+	333	WP_012536651.1	hypothetical protein	-
K7B00_RS11495 (K7B00_11495)	2264891..2265238	+	348	WP_012536650.1	IS66 family insertion sequence element accessory protein TnpB	-
K7B00_RS11500 (K7B00_11500)	2265288..2266925	+	1638	WP_180830442.1	IS66-like element ISAfe4 family transposase	-
K7B00_RS11505 (K7B00_11505)	2266970..2267164	+	195	WP_012536648.1	hypothetical protein	-
K7B00_RS11510 (K7B00_11510)	2267581..2268348	-	768	WP_012536647.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-46)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5323.25 Da
• Isoelectric Point: 10.0955

>T214447 WP_012536654.1 NZ_CP082238:c2263885-2263739 [Acidithiobacillus ferrooxidans]
MRIHYGPGYRVYFKRFGLELVVLLAGGDKSTQSADIALALKLARQLEG

Nucleotide

• Length: 147 bp

>T214447 NZ_CP110075:c61639-61370 [Enterococcus faecalis]
ATGTCGTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10699.15 Da
• Isoelectric Point: 5.5858

>AT214447 WP_012536655.1 NZ_CP082238:c2263735-2263436 [Acidithiobacillus ferrooxidans]
MEKLELHTWDSAEHLKTEEDIAAYLDACFEEAGDDAAFIARALGTVARARGMTRLAHDAGMGRESLYKALSGEGNPSFAT
ILKVAKALGVRIRAEALHV

Nucleotide

• Length: 300 bp

>AT214447 NZ_CP110075:c61866-61639 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA