Detailed information of TA system TA214446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1874225..1874862
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K7B00_RS09600
• Protein ID: WP_012536995.1
• Coordinates: 1874225..1874635 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J4Y7
• Locus tag: K7B00_RS09605
• Protein ID: WP_009567455.1
• Coordinates: 1874632..1874862 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS09580 (K7B00_09580)	1870805..1871872	-	1068	WP_225487051.1	phytoene/squalene synthase family protein	-
K7B00_RS09585 (K7B00_09585)	1872007..1873041	-	1035	WP_012536992.1	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-
K7B00_RS09590 (K7B00_09590)	1873045..1873494	-	450	WP_012536993.1	protein-export chaperone SecB	-
K7B00_RS09600 (K7B00_09600)	1874225..1874635	-	411	WP_012536995.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K7B00_RS09605 (K7B00_09605)	1874632..1874862	-	231	WP_009567455.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K7B00_RS16775	1875014..1875136	+	123	WP_257246597.1	hypothetical protein	-
K7B00_RS09610 (K7B00_09610)	1875165..1876685	-	1521	WP_012536996.1	aldehyde dehydrogenase family protein	-
K7B00_RS09615 (K7B00_09615)	1876988..1878700	+	1713	WP_009566247.1	proline--tRNA ligase	-
K7B00_RS09620 (K7B00_09620)	1878687..1879169	+	483	WP_009566245.1	universal stress protein	-
K7B00_RS09625 (K7B00_09625)	1879182..1879805	-	624	WP_225487052.1	ABC-type transport auxiliary lipoprotein family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15375.79 Da
• Isoelectric Point: 8.0756

>T214446 WP_012536995.1 NZ_CP082238:c1874635-1874225 [Acidithiobacillus ferrooxidans]
VIKYLLDTNICIFTIKNKPPEIREQFRQHHGQMAISTVTLMELIYGAEKSQVPERNLAEVEGFAARLEVLEFGSEAAKHT
GQLRAELAKIGKPIGPYDQMIAGHARSLGLFVVTNNLREFERVPGLRVMDWQSSRL

Nucleotide

• Length: 411 bp

>T214446 NZ_CP110074:2789482-2789625 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8573.77 Da
• Isoelectric Point: 5.0508

>AT214446 WP_009567455.1 NZ_CP082238:c1874862-1874632 [Acidithiobacillus ferrooxidans]
MEHATVFQINRSQAIRLPKSIAFPADVKRVEVVAIGRMRILVPAGESWESWFDGEGVSADFMESREQPAGQFREPL

Nucleotide

• Length: 231 bp

>AT214446 NZ_CP110074:c2789819-2789557 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Antitoxin

• AlphaFold DB: B7J4Y7