Detailed information of TA system TA214444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1380924..1381687
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B7J9L5
• Locus tag: K7B00_RS07170
• Protein ID: WP_009565062.1
• Coordinates: 1380924..1381418 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B7J9L6
• Locus tag: K7B00_RS07175
• Protein ID: WP_012536505.1
• Coordinates: 1381415..1381687 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS07135 (K7B00_07135)	1376689..1377471	+	783	WP_012607057.1	ATP-binding protein	-
K7B00_RS07140 (K7B00_07140)	1377464..1378306	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
K7B00_RS07150 (K7B00_07150)	1379024..1379530	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	-
K7B00_RS07155 (K7B00_07155)	1379527..1379856	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	-
K7B00_RS07160 (K7B00_07160)	1380143..1380286	+	144	WP_009569579.1	hypothetical protein	-
K7B00_RS07165 (K7B00_07165)	1380313..1380579	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
K7B00_RS07170 (K7B00_07170)	1380924..1381418	-	495	WP_009565062.1	GNAT family N-acetyltransferase	Toxin
K7B00_RS07175 (K7B00_07175)	1381415..1381687	-	273	WP_012536505.1	DUF1778 domain-containing protein	Antitoxin
K7B00_RS07180 (K7B00_07180)	1381768..1381980	-	213	WP_009562915.1	hypothetical protein	-
K7B00_RS07185 (K7B00_07185)	1382148..1382360	-	213	WP_041645801.1	hypothetical protein	-
K7B00_RS07190 (K7B00_07190)	1382764..1383585	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
K7B00_RS07195 (K7B00_07195)	1383826..1384071	+	246	WP_009568482.1	DUF2934 domain-containing protein	-
K7B00_RS07200 (K7B00_07200)	1384218..1385402	+	1185	WP_225487432.1	glycosyltransferase family 1 protein	-
K7B00_RS07205 (K7B00_07205)	1385436..1386650	+	1215	WP_012536508.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18013.83 Da
• Isoelectric Point: 10.4415

>T214444 WP_009565062.1 NZ_CP082238:c1381418-1380924 [Acidithiobacillus ferrooxidans]
MSLKLSAPQPLAAVHLLGDFESGEPALDEWLKRRAMNNQLTGASRTFVVVDEENRVRGFYAMAAGAVAHQVATSSVRRNM
PDPVPVMVLGRLAVDSRAQGIKLGAAMLQDAVKRAITVSQNTGVRALLVHALHERAKQFYEHYGFQESPQHPMTLMLRLN
SIKA

Nucleotide

• Length: 495 bp

>T214444 NZ_CP110074:2789111-2789251 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10071.52 Da
• Isoelectric Point: 5.0914

>AT214444 WP_012536505.1 NZ_CP082238:c1381687-1381415 [Acidithiobacillus ferrooxidans]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVLLDQVFFGLDAEKFRQFTAMLDAPPRGNEGLERLMA
VKAPWESNQA

Nucleotide

• Length: 273 bp

>AT214444 NZ_CP110074:c2789385-2789184 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: B7J9L5

Antitoxin

• AlphaFold DB: B7J9L6