Detailed information of TA system TA214439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 501377..502017
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K7B00_RS02700
• Protein ID: WP_009567232.1
• Coordinates: 501607..502017 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: B7J4M0
• Locus tag: K7B00_RS02695
• Protein ID: WP_009567231.1
• Coordinates: 501377..501607 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS02680 (K7B00_02680)	497255..498385	+	1131	WP_012536159.1	threonine synthase	-
K7B00_RS02685 (K7B00_02685)	498385..499350	+	966	WP_009567244.1	hypothetical protein	-
K7B00_RS02690 (K7B00_02690)	499351..501168	+	1818	WP_012536160.1	DHHA1 domain-containing protein	-
K7B00_RS02695 (K7B00_02695)	501377..501607	+	231	WP_009567231.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K7B00_RS02700 (K7B00_02700)	501607..502017	+	411	WP_009567232.1	type II toxin-antitoxin system VapC family toxin	Toxin
K7B00_RS02705 (K7B00_02705)	502150..503248	+	1099	WP_012606567.1	peptide chain release factor 2	-
K7B00_RS02710 (K7B00_02710)	503288..504775	+	1488	WP_229129796.1	lysine--tRNA ligase	-
K7B00_RS02715 (K7B00_02715)	504772..506016	+	1245	WP_012536161.1	lipoprotein-releasing ABC transporter permease subunit	-
K7B00_RS02720 (K7B00_02720)	506009..506704	+	696	WP_009566877.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15278.46 Da
• Isoelectric Point: 7.3848

>T214439 WP_009567232.1 NZ_CP082238:501607-502017 [Acidithiobacillus ferrooxidans]
MTLRYLLDTNICIYIAKHHPPAVRERFVQHGADDLAMSVITLGELCWGAEKSQARDKALASIQRLQERIQVAPLTPQVGI
HYGQIRAALESGGRPIGNNDLWIAAHARAEGWIVVTNNEREFRRVEGLQVENWVTA

Nucleotide

• Length: 411 bp

>T214439 NZ_CP110074:c2645134-2645033 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.76 Da
• Isoelectric Point: 4.4217

>AT214439 WP_009567231.1 NZ_CP082238:501377-501607 [Acidithiobacillus ferrooxidans]
MAIARIFQSGNSQAVRLPKEFRFEVDQVEIFRRGNEIILREHPVDGTGVFDALADLPEDFMSDGRQDIPPQARENF

Nucleotide

• Length: 231 bp

>AT214439 NZ_CP110074:2644909-2645088 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Structures

Antitoxin

• AlphaFold DB: B7J4M0