Detailed information of TA system TA214436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-HicB
• Location: 786492..787124
• Replicon: chromosome
• Accession: NZ_CP082237
• Organism: Caldalkalibacillus thermarum TA2.A1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: F5L789
• Locus tag: HUR95_RS04035
• Protein ID: WP_007504757.1
• Coordinates: 786774..787124 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HUR95_RS04030
• Protein ID: WP_007504756.1
• Coordinates: 786492..786770 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR95_RS04010 (HUR95_04010)	782582..783343	-	762	WP_007504751.1	rhomboid family intramembrane serine protease	-
HUR95_RS04015 (HUR95_04015)	783523..783897	+	375	WP_007504753.1	holo-ACP synthase	-
HUR95_RS04020 (HUR95_04020)	783987..784997	+	1011	WP_222822982.1	outer membrane lipoprotein-sorting protein	-
HUR95_RS04025 (HUR95_04025)	785140..786330	+	1191	WP_007504755.1	alanine racemase	-
HUR95_RS04030 (HUR95_04030)	786492..786770	+	279	WP_007504756.1	CopG family ribbon-helix-helix protein	Antitoxin
HUR95_RS04035 (HUR95_04035)	786774..787124	+	351	WP_007504757.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUR95_RS04040 (HUR95_04040)	787293..787760	+	468	WP_007504758.1	anti-sigma B factor RsbW	-
HUR95_RS04045 (HUR95_04045)	787729..788520	+	792	WP_007504759.1	RNA polymerase sigma factor SigB	-
HUR95_RS04050 (HUR95_04050)	788566..790740	+	2175	WP_042685259.1	Tex family protein	-
HUR95_RS04055 (HUR95_04055)	790718..790855	-	138	WP_007504761.1	cortex morphogenetic protein CmpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12906.02 Da
• Isoelectric Point: 5.1342

>T214436 WP_007504757.1 NZ_CP082237:786774-787124 [Caldalkalibacillus thermarum TA2.A1]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKMYGFEKDSVILLEQI
RTIDKQRLTDKITHLDEEMMARVDDALQISLGLIDF

Nucleotide

• Length: 351 bp

>T214436 NZ_CP110074:412893-413201 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10686.41 Da
• Isoelectric Point: 10.3581

>AT214436 WP_007504756.1 NZ_CP082237:786492-786770 [Caldalkalibacillus thermarum TA2.A1]
VSNSKTKRIMITLPQNLLKEVDGVAKKEQSNRSELIRQAMKFYLRERKKRQLRESMKKGYMEMANINLNMASEAFLAEEE
ADHTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT214436 NZ_CP110074:412619-412891 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: F5L789